Detailed information of TA system TA073133

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 951734..952375
• Replicon: chromosome
• Accession: NZ_CP019668
• Organism: Burkholderia cenocepacia strain VC7848

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B1K4X0
• Locus tag: A8E88_RS04295
• Protein ID: WP_006479524.1
• Coordinates: 951734..952072 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: A8E88_RS04300
• Protein ID: WP_006490972.1
• Coordinates: 952076..952375 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8E88_RS04280	948252..949685	-	1434	WP_012338512.1	aldehyde dehydrogenase family protein	-
A8E88_RS04285	949714..951354	-	1641	WP_011548230.1	acetolactate synthase large subunit	-
A8E88_RS04295	951734..952072	+	339	WP_006479524.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A8E88_RS04300	952076..952375	+	300	WP_006490972.1	putative addiction module antidote protein	Antitoxin
A8E88_RS04305	952424..953710	-	1287	WP_012338513.1	DUF445 domain-containing protein	-
A8E88_RS04310	953862..955082	-	1221	WP_012338514.1	FAD-dependent oxidoreductase	-
A8E88_RS04315	955072..955398	-	327	WP_006479527.1	non-heme iron oxygenase ferredoxin subunit	-
A8E88_RS04320	955434..955910	-	477	WP_006490977.1	aromatic-ring-hydroxylating dioxygenase subunit beta	-
A8E88_RS04325	955925..957196	-	1272	WP_027809065.1	Rieske 2Fe-2S domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (26-98)

Antitoxin

• Uncharacterized protein HI1420 (4-96)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12604.47 Da
• Isoelectric Point: 8.9594

>T73133 WP_006479524.1 NZ_CP019668:951734-952072 [Burkholderia cenocepacia]
MVDILQSVPYSAPTFSIRTTDVFEAWFAGLPDRVARRRIQARIDRLSMGNPGDWKSAGSPVVEMRIDHGPGYRVYYVRRG
TIWVILLCGGDKSTQQADIRAAHAMLAHLDME

Nucleotide

• Length: 339 bp

>T73133 NZ_CP019668:951734-952072 [Burkholderia cenocepacia]
ATGGTTGACATCCTGCAATCCGTGCCCTACAGTGCGCCTACGTTCAGCATCCGGACCACCGACGTCTTCGAAGCGTGGTT
CGCCGGCCTGCCGGATCGCGTCGCGCGGCGGCGCATCCAGGCGCGCATCGACCGTCTGTCGATGGGCAATCCGGGCGACT
GGAAATCCGCCGGCTCCCCCGTCGTCGAAATGCGTATCGACCACGGCCCCGGCTATCGCGTCTATTACGTGAGGCGCGGA
ACGATATGGGTCATCCTGCTCTGCGGCGGCGATAAGTCGACGCAACAGGCCGACATCCGCGCCGCGCACGCAATGCTCGC
GCACCTCGACATGGAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10608.04 Da
• Isoelectric Point: 6.4771

>AT73133 WP_006490972.1 NZ_CP019668:952076-952375 [Burkholderia cenocepacia]
MDKIKTRPWDSAEHLKTEDDMADYFDACLQEAGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAAA

Nucleotide

• Length: 300 bp

>AT73133 NZ_CP019668:952076-952375 [Burkholderia cenocepacia]
ATGGACAAGATCAAGACGCGGCCGTGGGATTCGGCCGAACACCTGAAAACCGAAGACGACATGGCCGACTACTTCGACGC
CTGCCTGCAGGAGGCCGGCGACGATCCGGCCTTCATCGCGCATGCGCTCGGCGTGATCGCCCGTGCCCGCGGCATGTCGC
AGGTCGCCCGCGATGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATCCTGAAGGTCATCAAGGCGCTCGGCCTGCAATTGCACGGCGCCAAGGCTGCCGCGTGA

Structures

Toxin

• AlphaFold DB: B1K4X0