Detailed information of TA system TA073126

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 478532..479158
• Replicon: chromosome
• Accession: NZ_CP019659
• Organism: Paenibacillus larvae subsp. larvae strain Eric_IV

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: V9W2Z1
• Locus tag: ERICIV_RS02440
• Protein ID: WP_023485406.1
• Coordinates: 478808..479158 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: ERICIV_RS02435
• Protein ID: WP_172423825.1
• Coordinates: 478532..478804 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ERICIV_RS02410	473737..474225	+	489	WP_077996651.1	hypothetical protein	-
ERICIV_RS02415	474243..475006	-	764	Protein_448	IS5 family transposase	-
ERICIV_RS02425	475677..476933	+	1257	WP_077996653.1	outer membrane lipoprotein carrier protein LolA	-
ERICIV_RS02430	477101..478288	+	1188	WP_077996654.1	alanine racemase	-
ERICIV_RS02435	478532..478804	+	273	WP_172423825.1	ribbon-helix-helix protein, CopG family	Antitoxin
ERICIV_RS02440	478808..479158	+	351	WP_023485406.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ERICIV_RS02445	479330..481534	+	2205	WP_104932789.1	RNA-binding transcriptional accessory protein	-
ERICIV_RS02450	481736..482302	-	567	WP_077997616.1	sigma-70 family RNA polymerase sigma factor	-
ERICIV_RS02455	482700..483158	+	459	WP_023485404.1	hypothetical protein	-
ERICIV_RS02460	483243..483470	+	228	WP_024093128.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-42)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12877.80 Da
• Isoelectric Point: 5.1393

>T73126 WP_023485406.1 NZ_CP019659:478808-479158 [Paenibacillus larvae subsp. larvae]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAETHGFDRDSVILLEQI
RTIDKQRLTDKITHLDEDTMRKVNDALQVSVGLIEF

Nucleotide

• Length: 351 bp

>T73126 NZ_CP019659:478808-479158 [Paenibacillus larvae subsp. larvae]
TTGATTGTCAAACGTGGTGATGTTTTCTTTGCGGATCTTTCTCCTGTTGTCGGTTCCGAGCAGGGGGGAGTGAGGCCTGT
TCTTATCATTCAGAACGATATCGGGAACCGGTTTAGCCCGACGGTAATCGTGGCGGCGATTACCGCCCAAATTCAAAAAG
CCAAGCTGCCCACTCATGTGGAGATCGATGCGGAAACACACGGATTTGACCGCGATTCGGTTATCTTGCTGGAACAGATC
CGGACCATTGACAAGCAGCGGTTGACCGATAAAATTACCCATTTAGATGAAGATACCATGCGTAAAGTGAACGATGCTTT
ACAGGTAAGTGTAGGGTTGATAGAATTTTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10556.15 Da
• Isoelectric Point: 7.2048

>AT73126 WP_172423825.1 NZ_CP019659:478532-478804 [Paenibacillus larvae subsp. larvae]
MHNTKRIMISLPDHLLKEVDGIVEKENSNRSEFIRQAMRLYLLERKKRTLRESMQRGYMEMAKINLNMASEAFQAEEEAD
NTVDRLVSGV

Nucleotide

• Length: 273 bp

>AT73126 NZ_CP019659:478532-478804 [Paenibacillus larvae subsp. larvae]
ATGCATAATACGAAGCGTATCATGATTAGTCTTCCAGACCACCTTCTGAAGGAGGTGGACGGCATCGTCGAGAAAGAAAA
CTCGAATCGCAGCGAGTTCATTCGACAAGCCATGAGATTGTACCTGCTCGAAAGAAAGAAGAGGACCCTCCGTGAATCCA
TGCAGAGAGGCTATATGGAAATGGCGAAAATTAATTTAAATATGGCGTCCGAAGCTTTTCAAGCGGAGGAAGAAGCCGAC
AATACGGTGGACCGCTTAGTGAGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A2L1TT28