Detailed information of TA system TA073125

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 23577..24142
• Replicon: plasmid unnamed2
• Accession: NZ_CP019657
• Organism: Paenibacillus larvae subsp. larvae strain Eric_III

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: ERICIII_RS23870
• Protein ID: WP_077997739.1
• Coordinates: 23813..24142 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: ERICIII_RS23865
• Protein ID: WP_077997741.1
• Coordinates: 23577..23816 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ERICIII_RS25055	19604..19807	+	204	WP_158672847.1	winged helix-turn-helix transcriptional regulator	-
ERICIII_RS23845	19921..20181	-	261	Protein_21	transposase	-
ERICIII_RS23850	20362..21819	+	1458	WP_077997736.1	SIR2 family protein	-
ERICIII_RS23855	21857..22531	+	675	WP_077997737.1	TIR domain-containing protein	-
ERICIII_RS23860	22692..22943	-	252	WP_077997738.1	hypothetical protein	-
ERICIII_RS25060	22951..23097	-	147	WP_155121122.1	hypothetical protein	-
ERICIII_RS23865	23577..23816	+	240	WP_077997741.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ERICIII_RS23870	23813..24142	+	330	WP_077997739.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ERICIII_RS23875	24387..25025	-	639	WP_077997682.1	hemolysin III family protein	-
ERICIII_RS23885	25793..26071	-	279	WP_077997683.1	HU family DNA-binding protein	-
ERICIII_RS23890	26181..26387	-	207	WP_077997684.1	cold-shock protein	-
ERICIII_RS23895	26744..27427	+	684	WP_077996502.1	helix-turn-helix domain-containing protein	-
ERICIII_RS23900	27391..28317	+	927	Protein_32	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..60868	60868

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-108)

Antitoxin

• Antitoxin PemI-like (1-79)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12407.45 Da
• Isoelectric Point: 7.3587

>T73125 WP_077997739.1 NZ_CP019657:23813-24142 [Paenibacillus larvae subsp. larvae]
VTVVPDRGDIVYMDFNPQSGHEQAGWRPALVLSPKLFNATTNLAVVCPITSRIKGYLFEVHLPDAMMTKGVILVHQLKSA
DWRSRRLEIREKAPQEIIDEVIEKIHTFL

Nucleotide

• Length: 330 bp

>T73125 NZ_CP019657:23813-24142 [Paenibacillus larvae subsp. larvae]
GTGACAGTTGTTCCAGATCGCGGTGATATCGTTTATATGGACTTTAATCCACAGAGCGGACATGAACAAGCCGGGTGGCG
ACCAGCCCTTGTACTGTCACCAAAATTATTTAATGCTACAACGAATCTTGCTGTAGTCTGCCCTATCACTTCTAGAATTA
AAGGCTACTTATTTGAAGTTCATCTTCCAGACGCAATGATGACAAAAGGTGTTATACTTGTGCACCAATTGAAAAGCGCC
GATTGGAGATCGCGGAGATTAGAAATTAGGGAGAAAGCACCACAAGAAATTATTGACGAAGTGATTGAAAAGATTCATAC
TTTCCTATAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8957.25 Da
• Isoelectric Point: 5.6987

>AT73125 WP_077997741.1 NZ_CP019657:23577-23816 [Paenibacillus larvae subsp. larvae]
MSTLVSKWGNSLALRIPRHFAESLNLNEGSEVELKLTEDCLSVKPKKRKYTLEELCASIKDGNQHDLIDFGKPVGEEIW

Nucleotide

• Length: 240 bp

>AT73125 NZ_CP019657:23577-23816 [Paenibacillus larvae subsp. larvae]
ATGAGCACGCTTGTAAGCAAATGGGGAAATAGTCTGGCACTTCGTATTCCCAGACATTTTGCTGAAAGCTTAAATCTTAA
TGAAGGCTCTGAAGTTGAATTAAAGCTAACAGAAGACTGCTTGAGTGTCAAGCCGAAGAAGAGAAAGTATACCCTTGAGG
AACTTTGTGCTTCTATTAAAGACGGTAATCAGCATGACCTTATTGATTTCGGTAAACCTGTTGGGGAGGAAATTTGGTGA