Detailed information of TA system TA073119

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 3818535..3819161
• Replicon: chromosome
• Accession: NZ_CP019651
• Organism: Paenibacillus larvae subsp. larvae strain ERIC_I

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: V9W2Z1
• Locus tag: ERICI_RS19855
• Protein ID: WP_023485406.1
• Coordinates: 3818535..3818885 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: ERICI_RS19860
• Protein ID: WP_172423825.1
• Coordinates: 3818889..3819161 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ERICI_RS19835	3813716..3813943	-	228	WP_036655907.1	hypothetical protein	-
ERICI_RS19840	3814535..3814993	-	459	WP_023485404.1	hypothetical protein	-
ERICI_RS19845	3815391..3815957	+	567	WP_036657428.1	sigma-70 family RNA polymerase sigma factor	-
ERICI_RS19850	3816159..3818363	-	2205	WP_046655196.1	RNA-binding transcriptional accessory protein	-
ERICI_RS19855	3818535..3818885	-	351	WP_023485406.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ERICI_RS19860	3818889..3819161	-	273	WP_172423825.1	ribbon-helix-helix protein, CopG family	Antitoxin
ERICI_RS19865	3819405..3820592	-	1188	WP_023485408.1	alanine racemase	-
ERICI_RS19870	3820760..3822016	-	1257	WP_024093122.1	outer membrane lipoprotein carrier protein LolA	-
ERICI_RS19880	3822561..3822767	-	207	WP_144029578.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-42)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12877.80 Da
• Isoelectric Point: 5.1393

>T73119 WP_023485406.1 NZ_CP019651:c3818885-3818535 [Paenibacillus larvae subsp. larvae]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAETHGFDRDSVILLEQI
RTIDKQRLTDKITHLDEDTMRKVNDALQVSVGLIEF

Nucleotide

• Length: 351 bp

>T73119 NZ_CP019651:c3818885-3818535 [Paenibacillus larvae subsp. larvae]
TTGATTGTCAAACGTGGTGATGTTTTCTTTGCGGATCTTTCTCCTGTTGTCGGTTCCGAGCAGGGGGGAGTGAGGCCTGT
TCTTATCATTCAGAACGATATCGGGAACCGGTTTAGCCCGACGGTAATCGTGGCGGCGATTACCGCCCAAATTCAAAAAG
CCAAGCTGCCCACTCATGTGGAGATCGATGCGGAAACACACGGATTTGACCGCGATTCGGTTATCTTGCTGGAACAGATC
CGGACCATTGACAAGCAGCGGTTGACCGATAAAATTACCCATTTAGATGAAGATACCATGCGTAAAGTGAACGATGCTTT
ACAGGTAAGTGTAGGGTTGATAGAATTTTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10556.15 Da
• Isoelectric Point: 7.2048

>AT73119 WP_172423825.1 NZ_CP019651:c3819161-3818889 [Paenibacillus larvae subsp. larvae]
MHNTKRIMISLPDHLLKEVDGIVEKENSNRSEFIRQAMRLYLLERKKRTLRESMQRGYMEMAKINLNMASEAFQAEEEAD
NTVDRLVSGV

Nucleotide

• Length: 273 bp

>AT73119 NZ_CP019651:c3819161-3818889 [Paenibacillus larvae subsp. larvae]
ATGCATAATACGAAGCGTATCATGATTAGTCTTCCAGACCACCTTCTGAAGGAGGTGGACGGCATCGTCGAGAAAGAAAA
CTCGAATCGCAGCGAGTTCATTCGACAAGCCATGAGATTGTACCTGCTCGAAAGAAAGAAGAGGACCCTCCGTGAATCCA
TGCAGAGAGGCTATATGGAAATGGCGAAAATTAATTTAAATATGGCGTCCGAAGCTTTTCAAGCGGAGGAAGAAGCCGAC
AATACGGTGGACCGCTTAGTGAGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A2L1TT28