Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3042012..3042666 Replicon chromosome
Accession NZ_CP019629
Organism Escherichia coli strain MGY

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag BWL12_RS15855 Protein ID WP_000244777.1
Coordinates 3042012..3042419 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag BWL12_RS15860 Protein ID WP_000354046.1
Coordinates 3042400..3042666 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWL12_RS15835 3037969..3039702 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
BWL12_RS15840 3039708..3040418 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
BWL12_RS15845 3040443..3041339 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
BWL12_RS15850 3041451..3041972 + 522 WP_001055874.1 flavodoxin FldB -
BWL12_RS15855 3042012..3042419 - 408 WP_000244777.1 toxin CptA Toxin
BWL12_RS15860 3042400..3042666 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
BWL12_RS15865 3042909..3043889 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
BWL12_RS15870 3044085..3044744 - 660 WP_000250274.1 hemolysin III family protein -
BWL12_RS15875 3044908..3045219 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
BWL12_RS15880 3045264..3046697 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
BWL12_RS15885 3046754..3047497 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T73090 WP_000244777.1 NZ_CP019629:c3042419-3042012 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T73090 NZ_CP019629:c3042419-3042012 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT73090 WP_000354046.1 NZ_CP019629:c3042666-3042400 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT73090 NZ_CP019629:c3042666-3042400 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References