Detailed information of TA system TA073058

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1512033..1512670
• Replicon: chromosome
• Accession: NZ_CP019626
• Organism: Bacillus sp. 275

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: BZ167_RS07200
• Protein ID: WP_003156187.1
• Coordinates: 1512033..1512383 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: BZ167_RS07205
• Protein ID: WP_003156188.1
• Coordinates: 1512389..1512670 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BZ167_RS07160	1507073..1507675	-	603	WP_007410234.1	SpoIIE family protein phosphatase	-
BZ167_RS07165	1507675..1508463	-	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
BZ167_RS07170	1508429..1508911	-	483	WP_007609591.1	anti-sigma B factor RsbW	-
BZ167_RS07175	1508908..1509237	-	330	WP_033575044.1	anti-sigma factor antagonist	-
BZ167_RS07180	1509301..1510308	-	1008	WP_015416875.1	PP2C family protein-serine/threonine phosphatase	-
BZ167_RS07185	1510320..1510721	-	402	WP_003156178.1	anti-sigma regulatory factor	-
BZ167_RS07190	1510724..1511089	-	366	WP_003156180.1	RsbT antagonist protein RsbS	-
BZ167_RS07195	1511094..1511915	-	822	WP_015416874.1	STAS domain-containing protein	-
BZ167_RS07200	1512033..1512383	-	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
BZ167_RS07205	1512389..1512670	-	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
BZ167_RS07210	1512790..1513959	-	1170	WP_015416873.1	alanine racemase	-
BZ167_RS07215	1514076..1515083	-	1008	WP_077392009.1	outer membrane lipoprotein carrier protein LolA	-
BZ167_RS07220	1515248..1515613	-	366	WP_007410229.1	holo-ACP synthase	-
BZ167_RS07225	1515706..1516305	+	600	WP_003156193.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T73058 WP_003156187.1 NZ_CP019626:c1512383-1512033 [Bacillus sp. 275]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T73058 NZ_CP019626:c1512383-1512033 [Bacillus sp. 275]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT73058 WP_003156188.1 NZ_CP019626:c1512670-1512389 [Bacillus sp. 275]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT73058 NZ_CP019626:c1512670-1512389 [Bacillus sp. 275]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5