Detailed information of TA system TA073056

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 4289829..4290358
• Replicon: chromosome
• Accession: NZ_CP019613
• Organism: Mycobacterium tuberculosis strain H112

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G0TMR4
• Locus tag: B0W98_RS20950
• Protein ID: WP_003411124.1
• Coordinates: 4289829..4290146 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: P9WJ74
• Locus tag: B0W98_RS20955
• Protein ID: WP_003411127.1
• Coordinates: 4290143..4290358 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0W98_RS20920	4284850..4285926	+	1077	WP_003411110.1	hypothetical protein	-
B0W98_RS20925	4285923..4286204	+	282	WP_003411112.1	hypothetical protein	-
B0W98_RS20930	4286240..4287313	+	1074	WP_003901338.1	quinone-dependent dihydroorotate dehydrogenase	-
B0W98_RS20935	4287318..4287848	-	531	WP_003411119.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
B0W98_RS20940	4287896..4289242	-	1347	WP_003411121.1	M20/M25/M40 family metallo-hydrolase	-
B0W98_RS20950	4289829..4290146	-	318	WP_003411124.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B0W98_RS20955	4290143..4290358	-	216	WP_003411127.1	antitoxin ParD2	Antitoxin
B0W98_RS20960	4290613..4291671	+	1059	WP_003411129.1	hypothetical protein	-
B0W98_RS20965	4291801..4292157	-	357	WP_003411130.1	hypothetical protein	-
B0W98_RS20970	4292252..4293034	-	783	WP_003411131.1	cell wall synthesis protein Wag31	-
B0W98_RS20975	4293302..4293592	-	291	WP_003900476.1	YggT family protein	-
B0W98_RS20980	4293754..4294410	-	657	WP_003411133.1	cell division protein SepF	-
B0W98_RS20985	4294476..4295252	-	777	WP_003411137.1	YggS family pyridoxal phosphate-dependent enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-92)

Antitoxin

• Conserved hypothetical protein CHP02574, addiction module (11-69)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12312.88 Da
• Isoelectric Point: 6.4831

>T73056 WP_003411124.1 NZ_CP019613:c4290146-4289829 [Mycobacterium tuberculosis]
MTRRLRVHNGVEDDLFEAFSYYADAAPDQIDRLYNLFVDAVTKRIPQAPNAFAPLFKHYRHIYLRPFRYYVAYRTTDEAI
DILAVRHGMENPNAVEAEISGRTFE

Nucleotide

• Length: 318 bp

>T73056 NZ_CP019613:c4290146-4289829 [Mycobacterium tuberculosis]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7880.70 Da
• Isoelectric Point: 4.4055

>AT73056 WP_003411127.1 NZ_CP019613:c4290358-4290143 [Mycobacterium tuberculosis]
VVVNRALLASVDALSRDEQIELVEHINGNLAEGMHISEANQALIEARANDTDDAHWSTIDDFDKRIRARLG

Nucleotide

• Length: 216 bp

>AT73056 NZ_CP019613:c4290358-4290143 [Mycobacterium tuberculosis]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Structures

Toxin

• AlphaFold DB: G0TMR4

Antitoxin

• AlphaFold DB: A0A7U4FAJ4