73052

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 4137703..4138344 Replicon chromosome
Accession NZ_CP019613
Organism Mycobacterium tuberculosis strain H112

Toxin (Protein)


Gene name vapC Uniprot ID P64926
Locus tag B0W98_RS20215 Protein ID WP_003410075.1
Coordinates 4137946..4138344 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WLM7
Locus tag B0W98_RS20210 Protein ID WP_003899127.1
Coordinates 4137703..4137945 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0W98_RS20200 4135757..4136101 - 345 WP_003410065.1 ferredoxin family protein -
B0W98_RS20205 4136290..4137615 - 1326 WP_003410070.1 DUF4143 domain-containing protein -
B0W98_RS20210 4137703..4137945 + 243 WP_003899127.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
B0W98_RS20215 4137946..4138344 + 399 WP_003410075.1 PIN domain nuclease Toxin
B0W98_RS20220 4138527..4138958 - 432 WP_003410078.1 MarR family winged helix-turn-helix transcriptional regulator -
B0W98_RS20225 4138999..4139493 + 495 WP_003410080.1 DUF1398 domain-containing protein -
B0W98_RS20240 4139930..4140109 - 180 Protein_3818 hypothetical protein -
B0W98_RS20245 4140170..4140817 + 648 WP_071854215.1 transposase -
B0W98_RS20250 4140771..4141361 + 591 WP_003899131.1 IS110 family transposase -
B0W98_RS20255 4141489..4142745 - 1257 WP_003902247.1 HNH endonuclease -
B0W98_RS20265 4143063..4143338 + 276 WP_016330411.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-120)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(7-48)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14731.02 Da        Isoelectric Point: 6.7067

>T73052 WP_003410075.1 NZ_CP019613:4137946-4138344 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Download         Length: 399 bp

>T73052 NZ_CP019613:4137946-4138344 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8851.73 Da        Isoelectric Point: 4.1621

>AT73052 WP_003899127.1 NZ_CP019613:4137703-4137945 [Mycobacterium tuberculosis]
VYSGVVSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Download         Length: 243 bp

>AT73052 NZ_CP019613:4137703-4137945 [Mycobacterium tuberculosis]
GTGTATAGTGGTGTTGTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACT
CGATTCCAAGCGAAGTGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGC
TGCAGGGCAGCGGTTTCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCG
TAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4CHG

Antitoxin

Source ID Structure
AlphaFold DB A0A045JPF9

References