Detailed information of TA system TA073046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 4069822..4070525
• Replicon: chromosome
• Accession: NZ_CP019613
• Organism: Mycobacterium tuberculosis strain H112

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: B0W98_RS19785
• Protein ID: WP_003409778.1
• Coordinates: 4069822..4070151 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: B0W98_RS19790
• Protein ID: WP_003409780.1
• Coordinates: 4070148..4070525 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0W98_RS19765	4066205..4067275	+	1071	WP_003902252.1	epoxide hydrolase EphB	-
B0W98_RS19770	4067272..4067787	+	516	WP_003409718.1	flavin reductase family protein	-
B0W98_RS19775	4067784..4068845	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
B0W98_RS19780	4068842..4069612	+	771	WP_003409775.1	SDR family oxidoreductase	-
B0W98_RS19785	4069822..4070151	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B0W98_RS19790	4070148..4070525	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
B0W98_RS19795	4070522..4071112	-	591	WP_024918653.1	SEC-C domain-containing protein	-
B0W98_RS19800	4071167..4072531	+	1365	WP_003903691.1	HNH endonuclease	-
B0W98_RS19805	4072686..4073138	-	453	WP_003899095.1	lipoprotein	-
B0W98_RS19810	4073202..4073603	+	402	WP_003409869.1	hypothetical protein	-
B0W98_RS19815	4073596..4073778	-	183	WP_003409870.1	hypothetical protein	-
B0W98_RS19820	4073892..4074242	-	351	WP_003409871.1	hypothetical protein	-
B0W98_RS19825	4074253..4075155	-	903	WP_003899097.1	hypothetical protein	-
B0W98_RS19830	4075176..4075367	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T73046 WP_003409778.1 NZ_CP019613:c4070151-4069822 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T73046 NZ_CP019613:c4070151-4069822 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT73046 WP_003409780.1 NZ_CP019613:c4070525-4070148 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT73046 NZ_CP019613:c4070525-4070148 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8