Detailed information of TA system TA073038

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3128244..3128812
• Replicon: chromosome
• Accession: NZ_CP019613
• Organism: Mycobacterium tuberculosis strain H112

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: B0W98_RS15420
• Protein ID: WP_003405865.1
• Coordinates: 3128438..3128812 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: B0W98_RS15415
• Protein ID: WP_003405863.1
• Coordinates: 3128244..3128441 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0W98_RS15395	3124285..3124923	-	639	WP_003898730.1	lipid droplet-associated protein	-
B0W98_RS15400	3125013..3126020	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
B0W98_RS15405	3126037..3127020	-	984	WP_003898731.1	hypothetical protein	-
B0W98_RS15410	3127083..3128156	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
B0W98_RS15415	3128244..3128441	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
B0W98_RS15420	3128438..3128812	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
B0W98_RS15425	3129015..3129713	+	699	WP_003898733.1	hypothetical protein	-
B0W98_RS15430	3129831..3130016	+	186	WP_003901093.1	hypothetical protein	-
B0W98_RS15435	3129943..3130218	-	276	WP_003405867.1	hypothetical protein	-
B0W98_RS15440	3130461..3130784	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
B0W98_RS15445	3130799..3131659	-	861	WP_003898734.1	hypothetical protein	-
B0W98_RS15450	3131692..3132462	-	771	Protein_2910	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T73038 WP_003405865.1 NZ_CP019613:3128438-3128812 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T73038 NZ_CP019613:3128438-3128812 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT73038 WP_003405863.1 NZ_CP019613:3128244-3128441 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT73038 NZ_CP019613:3128244-3128441 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07