Detailed information of TA system TA073035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 2901990..2902609
• Replicon: chromosome
• Accession: NZ_CP019613
• Organism: Mycobacterium tuberculosis strain H112

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: B0W98_RS14260
• Protein ID: WP_003404726.1
• Coordinates: 2902175..2902609 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: B0W98_RS14255
• Protein ID: WP_003898641.1
• Coordinates: 2901990..2902169 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0W98_RS14245	2897463..2899043	+	1581	WP_003910977.1	serine hydrolase	-
B0W98_RS14250	2899040..2901433	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
B0W98_RS21735	2901706..2901864	+	159	WP_003404720.1	hypothetical protein	-
B0W98_RS14255	2901990..2902169	+	180	WP_003898641.1	antitoxin	Antitoxin
B0W98_RS14260	2902175..2902609	+	435	WP_003404726.1	SRPBCC family protein	Toxin
B0W98_RS14265	2902707..2903480	+	774	WP_003404735.1	VOC family protein	-
B0W98_RS14270	2903545..2903994	+	450	WP_003404738.1	hypothetical protein	-
B0W98_RS14275	2904129..2904359	-	231	WP_003898642.1	hypothetical protein	-
B0W98_RS14280	2904526..2906034	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
B0W98_RS14285	2906036..2907274	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T73035 WP_003404726.1 NZ_CP019613:2902175-2902609 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T73035 NZ_CP019613:2902175-2902609 [Mycobacterium tuberculosis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT73035 WP_003898641.1 NZ_CP019613:2901990-2902169 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT73035 NZ_CP019613:2901990-2902169 [Mycobacterium tuberculosis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5