73022

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-CcdA
Location 2528891..2529567 Replicon chromosome
Accession NZ_CP019613
Organism Mycobacterium tuberculosis strain H112

Toxin (Protein)


Gene name vapC Uniprot ID G0TPQ9
Locus tag B0W98_RS12230 Protein ID WP_003898500.1
Coordinates 2528891..2529304 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TPR0
Locus tag B0W98_RS12235 Protein ID WP_003402915.1
Coordinates 2529301..2529567 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0W98_RS12200 2524236..2524538 - 303 WP_003402896.1 DUF3349 domain-containing protein -
B0W98_RS12205 2524598..2524876 - 279 WP_003402901.1 hypothetical protein -
B0W98_RS12210 2524873..2526126 - 1254 WP_003402904.1 inorganic phosphate transporter family protein -
B0W98_RS12215 2526246..2526632 - 387 WP_003402907.1 VOC family protein -
B0W98_RS12220 2526695..2527579 - 885 WP_003402909.1 SDR family oxidoreductase -
B0W98_RS12225 2527675..2528577 - 903 WP_003915439.1 1,4-dihydroxy-2-naphthoyl-CoA synthase -
B0W98_RS12230 2528891..2529304 - 414 WP_003898500.1 PIN domain-containing protein Toxin
B0W98_RS12235 2529301..2529567 - 267 WP_003402915.1 antitoxin VapB3 Antitoxin
B0W98_RS12240 2529759..2531474 - 1716 WP_003402917.1 fatty-acid--CoA ligase FadD8 -
B0W98_RS12245 2531552..2533156 + 1605 WP_003402920.1 amidohydrolase -
B0W98_RS12250 2533153..2534133 + 981 WP_003402923.1 o-succinylbenzoate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Pae0151-like, PIN domain

(13-129)

Antitoxin

Post-segregation antitoxin CcdA

(19-61)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 14667.78 Da        Isoelectric Point: 7.3603

>T73022 WP_003898500.1 NZ_CP019613:c2529304-2528891 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Download         Length: 414 bp

>T73022 NZ_CP019613:c2529304-2528891 [Mycobacterium tuberculosis]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9543.79 Da        Isoelectric Point: 4.5994

>AT73022 WP_003402915.1 NZ_CP019613:c2529567-2529301 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Download         Length: 267 bp

>AT73022 NZ_CP019613:c2529567-2529301 [Mycobacterium tuberculosis]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TPQ9

Antitoxin

Source ID Structure
AlphaFold DB G0TPR0

References