Detailed information of TA system TA073013

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 1244080..1244609
• Replicon: chromosome
• Accession: NZ_CP019613
• Organism: Mycobacterium tuberculosis strain H112

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: B0W98_RS06210
• Protein ID: WP_003417760.1
• Coordinates: 1244352..1244609 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: B0W98_RS06205
• Protein ID: WP_003417757.1
• Coordinates: 1244080..1244355 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0W98_RS06170	1240653..1241447	-	795	WP_003417738.1	BBE domain-containing protein	-
B0W98_RS06175	1241529..1241900	-	372	WP_003417739.1	FAD-binding oxidoreductase	-
B0W98_RS21555	1241798..1242016	-	219	WP_157132559.1	hypothetical protein	-
B0W98_RS06185	1242043..1242303	-	261	WP_003417741.1	hypothetical protein	-
B0W98_RS06190	1242418..1242807	+	390	WP_003417745.1	DUF732 domain-containing protein	-
B0W98_RS06195	1242821..1243114	-	294	WP_003417749.1	DUF3017 domain-containing protein	-
B0W98_RS06200	1243111..1243956	-	846	WP_003417751.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
B0W98_RS06205	1244080..1244355	+	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
B0W98_RS06210	1244352..1244609	+	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
B0W98_RS06215	1244651..1245841	+	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
B0W98_RS06220	1245958..1246326	+	369	WP_003417765.1	FHA domain-containing protein	-
B0W98_RS06225	1246323..1246874	-	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
B0W98_RS06230	1246881..1247462	-	582	WP_003417769.1	ATP/GTP-binding protein	-
B0W98_RS06235	1247443..1247811	-	369	WP_003417772.1	DUF742 domain-containing protein	-
B0W98_RS06240	1247789..1248181	-	393	WP_003417776.1	roadblock/LC7 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T73013 WP_003417760.1 NZ_CP019613:1244352-1244609 [Mycobacterium tuberculosis]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T73013 NZ_CP019613:1244352-1244609 [Mycobacterium tuberculosis]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTGGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT73013 WP_003417757.1 NZ_CP019613:1244080-1244355 [Mycobacterium tuberculosis]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT73013 NZ_CP019613:1244080-1244355 [Mycobacterium tuberculosis]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Structures

Toxin

• PDB: 3OEI

Antitoxin

• AlphaFold DB: G0TI94