73006

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 586424..586994 Replicon chromosome
Accession NZ_CP019613
Organism Mycobacterium tuberculosis strain H112

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag B0W98_RS03110 Protein ID WP_003414166.1
Coordinates 586424..586780 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag B0W98_RS03115 Protein ID WP_003901465.1
Coordinates 586764..586994 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0W98_RS03090 581874..583562 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
B0W98_RS03095 583566..583892 - 327 WP_003414157.1 hypothetical protein -
B0W98_RS03100 584023..584652 + 630 WP_003414158.1 DUF3558 family protein -
B0W98_RS03105 584671..586322 + 1652 Protein_577 CocE/NonD family hydrolase -
B0W98_RS03110 586424..586780 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
B0W98_RS03115 586764..586994 - 231 WP_003901465.1 antitoxin MazE Antitoxin
B0W98_RS03120 587037..588080 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
B0W98_RS03125 588271..588546 + 276 WP_003911993.1 DUF1778 domain-containing protein -
B0W98_RS21520 588722..588976 - 255 WP_003917684.1 hypothetical protein -
B0W98_RS03135 589124..589528 + 405 WP_003414181.1 hypothetical protein -
B0W98_RS03140 589525..589716 + 192 WP_003414184.1 hypothetical protein -
B0W98_RS03145 589915..591069 + 1155 WP_003899488.1 DDE-type integrase/transposase/recombinase -
B0W98_RS03150 591303..591560 + 258 WP_003899489.1 hypothetical protein -
B0W98_RS03155 591665..591976 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T73006 WP_003414166.1 NZ_CP019613:c586780-586424 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T73006 NZ_CP019613:c586780-586424 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT73006 WP_003901465.1 NZ_CP019613:c586994-586764 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT73006 NZ_CP019613:c586994-586764 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4FBW3

References