Detailed information of TA system TA073000

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 346027..346658
• Replicon: chromosome
• Accession: NZ_CP019613
• Organism: Mycobacterium tuberculosis strain H112

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: B0W98_RS01745
• Protein ID: WP_003413174.1
• Coordinates: 346281..346658 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: B0W98_RS01740
• Protein ID: WP_003413167.1
• Coordinates: 346027..346284 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0W98_RS01705	341849..342256	+	408	WP_003413029.1	hypothetical protein	-
B0W98_RS01710	342552..343763	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
B0W98_RS01720	343890..344549	+	660	WP_003902296.1	hypothetical protein	-
B0W98_RS01725	344546..345208	+	663	WP_003905878.1	hypothetical protein	-
B0W98_RS01730	345205..345482	+	278	Protein_318	type II toxin-antitoxin system VapB family antitoxin	-
B0W98_RS01735	345575..345988	+	414	WP_003413164.1	PIN domain nuclease	-
B0W98_RS01740	346027..346284	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
B0W98_RS01745	346281..346658	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
B0W98_RS01750	346674..347048	-	375	WP_003413177.1	hypothetical protein	-
B0W98_RS01755	347148..347543	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
B0W98_RS01760	347540..347785	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
B0W98_RS01770	348196..348615	-	420	WP_003413190.1	A24 family peptidase	-
B0W98_RS01775	348627..349436	-	810	WP_003413193.1	shikimate dehydrogenase	-
B0W98_RS01780	349433..350686	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
B0W98_RS01785	350679..351191	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T73000 WP_003413174.1 NZ_CP019613:346281-346658 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T73000 NZ_CP019613:346281-346658 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT73000 WP_003413167.1 NZ_CP019613:346027-346284 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT73000 NZ_CP019613:346027-346284 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5