73

Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) Rv0298-Rv0299/RHH-MazF
Location 363252..363778 Replicon chromosome
Accession NC_000962
Organism Mycobacterium tuberculosis H37Rv

Toxin (Protein)


Gene name Rv0299 Uniprot ID O07226
Locus tag Rv0299 Protein ID NP_214813.1
Coordinates 363476..363778 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name Rv0298 Uniprot ID P9WJ09
Locus tag Rv0298 Protein ID NP_214812.1
Coordinates 363252..363479 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Rv0295c 358945..359748 - 804 NP_214809.1 hypothetical protein -
Rv0296c 359758..361155 - 1398 YP_177712.1 sulfatase -
Rv0297 361334..363109 + 1776 YP_177713.1 PE-PGRS family protein PE_PGRS5 -
Rv0298 363252..363479 + 228 NP_214812.1 antitoxin Antitoxin
Rv0299 363476..363778 + 303 NP_214813.1 toxin Toxin
Rv0300 363826..364047 + 222 NP_214814.1 antitoxin VapB2 -
Rv0301 364044..364469 + 426 NP_214815.1 ribonuclease VapC2 -
Rv0302 364605..365237 + 633 NP_214816.1 transcriptional regulator -
Rv0303 365234..366142 + 909 NP_214817.1 dehydrogenase/reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Ribbon-helix-helix protein, CopG

(6-41)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 10442.16 Da        Isoelectric Point: 4.9609

>T73 NP_214813.1 NC_000962:363476-363778 [Mycobacterium tuberculosis H37Rv]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Download         Length: 303 bp

>T73 NC_000962:363476-363778 [Mycobacterium tuberculosis H37Rv]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8130.21 Da        Isoelectric Point: 7.0084

>AT73 NP_214812.1 NC_000962:363252-363479 [Mycobacterium tuberculosis H37Rv]
MTKEKISVTVDAAVLAAIDADARAAGLNRSEMIEQALRNEHLRVALRDYTAKTVPALDIDAYAQRVYQANRAAGS

Download         Length: 228 bp

>AT73 NC_000962:363252-363479 [Mycobacterium tuberculosis H37Rv]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGGGCGGCCGGAAGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB O07226

Antitoxin

Source ID Structure
AlphaFold DB P9WJ09

References

(1) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113] experimental


experimental experimental literature