Detailed information of TA system TA072993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 3642835..3643364
• Replicon: chromosome
• Accession: NZ_CP019612
• Organism: Mycobacterium tuberculosis strain H107

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: B0W96_RS17795
• Protein ID: WP_003417760.1
• Coordinates: 3643107..3643364 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: B0W96_RS17790
• Protein ID: WP_003417757.1
• Coordinates: 3642835..3643110 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0W96_RS17755	3639408..3640202	-	795	WP_003417738.1	BBE domain-containing protein	-
B0W96_RS17760	3640284..3640655	-	372	WP_112612200.1	FAD-binding oxidoreductase	-
B0W96_RS21660	3640553..3640771	-	219	WP_157132559.1	hypothetical protein	-
B0W96_RS17770	3640798..3641061	-	264	WP_003906084.1	hypothetical protein	-
B0W96_RS17775	3641173..3641562	+	390	WP_003417745.1	DUF732 domain-containing protein	-
B0W96_RS17780	3641576..3641869	-	294	WP_003417749.1	DUF3017 domain-containing protein	-
B0W96_RS17785	3641866..3642711	-	846	WP_003417751.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
B0W96_RS17790	3642835..3643110	+	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
B0W96_RS17795	3643107..3643364	+	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
B0W96_RS17800	3643406..3644596	+	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
B0W96_RS17805	3644713..3645081	+	369	WP_003417765.1	FHA domain-containing protein	-
B0W96_RS17810	3645078..3645629	-	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
B0W96_RS17815	3645636..3646217	-	582	WP_003417769.1	ATP/GTP-binding protein	-
B0W96_RS17820	3646198..3646566	-	369	WP_003417772.1	DUF742 domain-containing protein	-
B0W96_RS17825	3646544..3646936	-	393	WP_003417776.1	roadblock/LC7 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T72993 WP_003417760.1 NZ_CP019612:3643107-3643364 [Mycobacterium tuberculosis]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T72993 NZ_CP019612:3643107-3643364 [Mycobacterium tuberculosis]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTGGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT72993 WP_003417757.1 NZ_CP019612:3642835-3643110 [Mycobacterium tuberculosis]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT72993 NZ_CP019612:3642835-3643110 [Mycobacterium tuberculosis]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Structures

Toxin

• PDB: 3OEI

Antitoxin

• PDB: 3CTO
• PDB: 3OEI
• PDB: 3D55
• AlphaFold DB: G0TI94