Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3050056..3050743 Replicon chromosome
Accession NZ_CP019612
Organism Mycobacterium tuberculosis strain H107

Toxin (Protein)


Gene name vapC Uniprot ID P65044
Locus tag B0W96_RS15055 Protein ID WP_003414624.1
Coordinates 3050300..3050743 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag B0W96_RS15050 Protein ID WP_003414620.1
Coordinates 3050056..3050313 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0W96_RS15030 3045376..3046230 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
B0W96_RS15035 3046286..3047449 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
B0W96_RS15040 3047466..3048680 - 1215 WP_003899518.1 zinc metalloprotease Rip -
B0W96_RS15045 3048688..3049929 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
B0W96_RS15050 3050056..3050313 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
B0W96_RS15055 3050300..3050743 + 444 WP_003414624.1 type II toxin-antitoxin system VapC family toxin Toxin
B0W96_RS15060 3050823..3051485 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
B0W96_RS15065 3051581..3051772 + 192 WP_003414632.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
B0W96_RS15070 3051765..3053852 + 2088 WP_003414635.1 cytochrome c biogenesis protein DipZ -
B0W96_RS15075 3053948..3054529 + 582 WP_003414644.1 fasciclin domain-containing protein -
B0W96_RS15080 3054581..3054895 + 315 WP_003899522.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16595.72 Da        Isoelectric Point: 6.4890

>T72990 WP_003414624.1 NZ_CP019612:3050300-3050743 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T72990 NZ_CP019612:3050300-3050743 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT72990 WP_003414620.1 NZ_CP019612:3050056-3050313 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT72990 NZ_CP019612:3050056-3050313 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VWO

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References