Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2735330..2735961 Replicon chromosome
Accession NZ_CP019612
Organism Mycobacterium tuberculosis strain H107

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag B0W96_RS13315 Protein ID WP_003413174.1
Coordinates 2735584..2735961 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag B0W96_RS13310 Protein ID WP_003413167.1
Coordinates 2735330..2735587 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0W96_RS13275 2731151..2731558 + 408 WP_003413029.1 hypothetical protein -
B0W96_RS13280 2731854..2733065 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
B0W96_RS13290 2733192..2733851 + 660 WP_003900846.1 LppA family lipoprotein -
B0W96_RS13295 2733848..2734510 + 663 WP_003900848.1 hypothetical protein -
B0W96_RS13300 2734507..2734785 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
B0W96_RS13305 2734878..2735291 + 414 WP_003413164.1 PIN domain nuclease -
B0W96_RS13310 2735330..2735587 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
B0W96_RS13315 2735584..2735961 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
B0W96_RS13320 2735977..2736351 - 375 WP_003413177.1 hypothetical protein -
B0W96_RS13325 2736451..2736846 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
B0W96_RS13330 2736843..2737088 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
B0W96_RS13340 2737499..2737918 - 420 WP_003413190.1 A24 family peptidase -
B0W96_RS13345 2737930..2738739 - 810 WP_003413193.1 shikimate dehydrogenase -
B0W96_RS13350 2738736..2739989 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
B0W96_RS13355 2739982..2740494 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T72980 WP_003413174.1 NZ_CP019612:2735584-2735961 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T72980 NZ_CP019612:2735584-2735961 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTGCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT72980 WP_003413167.1 NZ_CP019612:2735330-2735587 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT72980 NZ_CP019612:2735330-2735587 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References