Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 2123866..2124507 Replicon chromosome
Accession NZ_CP019612
Organism Mycobacterium tuberculosis strain H107

Toxin (Protein)


Gene name vapC Uniprot ID P64926
Locus tag B0W96_RS10330 Protein ID WP_003410075.1
Coordinates 2124109..2124507 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WLM7
Locus tag B0W96_RS10325 Protein ID WP_003899127.1
Coordinates 2123866..2124108 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0W96_RS10315 2121920..2122264 - 345 WP_003410065.1 ferredoxin family protein -
B0W96_RS10320 2122453..2123778 - 1326 WP_003410070.1 DUF4143 domain-containing protein -
B0W96_RS10325 2123866..2124108 + 243 WP_003899127.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
B0W96_RS10330 2124109..2124507 + 399 WP_003410075.1 PIN domain nuclease Toxin
B0W96_RS10335 2124690..2125121 - 432 WP_003410078.1 MarR family winged helix-turn-helix transcriptional regulator -
B0W96_RS10340 2125162..2125656 + 495 WP_003410080.1 DUF1398 domain-containing protein -
B0W96_RS10355 2126093..2126272 - 180 Protein_1959 hypothetical protein -
B0W96_RS10360 2126333..2126980 + 648 WP_071854215.1 transposase -
B0W96_RS10365 2126934..2127524 + 591 WP_003899131.1 IS110 family transposase -
B0W96_RS10370 2127652..2128908 - 1257 WP_003410095.1 HNH endonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-120)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(7-48)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14731.02 Da        Isoelectric Point: 6.7067

>T72972 WP_003410075.1 NZ_CP019612:2124109-2124507 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Download         Length: 399 bp

>T72972 NZ_CP019612:2124109-2124507 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8851.73 Da        Isoelectric Point: 4.1621

>AT72972 WP_003899127.1 NZ_CP019612:2123866-2124108 [Mycobacterium tuberculosis]
VYSGVVSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Download         Length: 243 bp

>AT72972 NZ_CP019612:2123866-2124108 [Mycobacterium tuberculosis]
GTGTATAGTGGTGTTGTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACT
CGATTCCAAGCGAAGTGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGC
TGCAGGGCAGCGGTTTCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCG
TAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4CHG

Antitoxin

Source ID Structure
PDB 4CHG
AlphaFold DB A0A045JPF9

References