Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2100121..2100707 Replicon chromosome
Accession NZ_CP019612
Organism Mycobacterium tuberculosis strain H107

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag B0W96_RS10230 Protein ID WP_003410010.1
Coordinates 2100121..2100465 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag B0W96_RS10235 Protein ID WP_003410014.1
Coordinates 2100459..2100707 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0W96_RS10190 2095827..2096426 + 600 WP_003409989.1 L-lysine exporter -
B0W96_RS10195 2096842..2097270 + 429 WP_003409992.1 cellulose-binding protein -
B0W96_RS10200 2097496..2098035 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
B0W96_RS10210 2098555..2099115 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
B0W96_RS10215 2099112..2099453 - 342 WP_003410003.1 DUF2384 domain-containing protein -
B0W96_RS10220 2099539..2099796 + 258 WP_003410006.1 hypothetical protein -
B0W96_RS10225 2099697..2100032 - 336 WP_003410009.1 dehydrogenase -
B0W96_RS10230 2100121..2100465 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
B0W96_RS10235 2100459..2100707 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
B0W96_RS10240 2100807..2103122 - 2316 WP_003899120.1 cation transporter ATPase CptG -
B0W96_RS10245 2103119..2103391 - 273 WP_003410017.1 DUF1490 family protein -
B0W96_RS10250 2103444..2103800 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
B0W96_RS10255 2103957..2104724 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T72971 WP_003410010.1 NZ_CP019612:c2100465-2100121 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T72971 NZ_CP019612:c2100465-2100121 [Mycobacterium tuberculosis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT72971 WP_003410014.1 NZ_CP019612:c2100707-2100459 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT72971 NZ_CP019612:c2100707-2100459 [Mycobacterium tuberculosis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References