Detailed information of TA system TA072966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2059102..2059805
• Replicon: chromosome
• Accession: NZ_CP019612
• Organism: Mycobacterium tuberculosis strain H107

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: B0W96_RS09930
• Protein ID: WP_003409778.1
• Coordinates: 2059102..2059431 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: B0W96_RS09935
• Protein ID: WP_003409780.1
• Coordinates: 2059428..2059805 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0W96_RS09910	2055485..2056555	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
B0W96_RS09915	2056552..2057067	+	516	WP_112612172.1	flavin reductase family protein	-
B0W96_RS09920	2057064..2058125	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
B0W96_RS09925	2058122..2058892	+	771	WP_003409775.1	SDR family oxidoreductase	-
B0W96_RS09930	2059102..2059431	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B0W96_RS09935	2059428..2059805	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
B0W96_RS09940	2059802..2060392	-	591	WP_003409784.1	SEC-C domain-containing protein	-
B0W96_RS09945	2060447..2061811	+	1365	WP_003903691.1	HNH endonuclease	-
B0W96_RS09950	2061966..2062418	-	453	WP_003899095.1	lipoprotein	-
B0W96_RS09955	2062482..2062883	+	402	WP_003409869.1	hypothetical protein	-
B0W96_RS09960	2062876..2063058	-	183	WP_003409870.1	hypothetical protein	-
B0W96_RS09965	2063172..2063522	-	351	WP_003899096.1	hypothetical protein	-
B0W96_RS09970	2063533..2064435	-	903	WP_003899097.1	hypothetical protein	-
B0W96_RS09975	2064456..2064647	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T72966 WP_003409778.1 NZ_CP019612:c2059431-2059102 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T72966 NZ_CP019612:c2059431-2059102 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT72966 WP_003409780.1 NZ_CP019612:c2059805-2059428 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT72966 NZ_CP019612:c2059805-2059428 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8