Detailed information of TA system TA072958

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1113937..1114505
• Replicon: chromosome
• Accession: NZ_CP019612
• Organism: Mycobacterium tuberculosis strain H107

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: B0W96_RS05530
• Protein ID: WP_003405865.1
• Coordinates: 1114131..1114505 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: B0W96_RS05525
• Protein ID: WP_003405863.1
• Coordinates: 1113937..1114134 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0W96_RS05505	1109978..1110616	-	639	WP_003898730.1	lipid droplet-associated protein	-
B0W96_RS05510	1110706..1111713	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
B0W96_RS05515	1111730..1112713	-	984	WP_003898731.1	hypothetical protein	-
B0W96_RS05520	1112776..1113849	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
B0W96_RS05525	1113937..1114134	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
B0W96_RS05530	1114131..1114505	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
B0W96_RS05535	1114708..1115406	+	699	WP_112612158.1	hypothetical protein	-
B0W96_RS05540	1115524..1115709	+	186	WP_003901093.1	hypothetical protein	-
B0W96_RS05545	1115636..1115911	-	276	WP_003405867.1	hypothetical protein	-
B0W96_RS05550	1116154..1116477	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
B0W96_RS05555	1116492..1117352	-	861	WP_003898734.1	hypothetical protein	-
B0W96_RS05560	1117385..1118155	-	771	Protein_1055	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T72958 WP_003405865.1 NZ_CP019612:1114131-1114505 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T72958 NZ_CP019612:1114131-1114505 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT72958 WP_003405863.1 NZ_CP019612:1113937-1114134 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT72958 NZ_CP019612:1113937-1114134 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07