Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3917349..3917953 Replicon chromosome
Accession NZ_CP019611
Organism Mycobacterium tuberculosis strain H83

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag B0W95_RS19105 Protein ID WP_003414492.1
Coordinates 3917349..3917741 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag B0W95_RS19110 Protein ID WP_003414495.1
Coordinates 3917738..3917953 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0W95_RS19075 3912499..3913221 - 723 WP_003912014.1 CRISPR-associated endoribonuclease Cas6 -
B0W95_RS19080 3913621..3914166 - 546 WP_003913758.1 DUF1802 family protein -
B0W95_RS19085 3914438..3915322 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
B0W95_RS19090 3915325..3916212 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
B0W95_RS19095 3916517..3917062 - 546 WP_003899500.1 DUF1802 family protein -
B0W95_RS19100 3917059..3917328 - 270 WP_003912856.1 DUF2277 family protein -
B0W95_RS19105 3917349..3917741 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
B0W95_RS19110 3917738..3917953 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
B0W95_RS19115 3918000..3918749 + 750 WP_003414497.1 enoyl-CoA hydratase -
B0W95_RS19120 3918828..3919910 - 1083 WP_003414499.1 ABC transporter ATP-binding protein -
B0W95_RS19125 3919903..3921213 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
B0W95_RS19130 3921216..3922043 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
B0W95_RS19135 3922040..3922951 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3879340..3919910 40570


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T72932 WP_003414492.1 NZ_CP019611:c3917741-3917349 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T72932 NZ_CP019611:c3917741-3917349 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT72932 WP_003414495.1 NZ_CP019611:c3917953-3917738 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT72932 NZ_CP019611:c3917953-3917738 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References