Detailed information of TA system TA072930

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3889305..3889875
• Replicon: chromosome
• Accession: NZ_CP019611
• Organism: Mycobacterium tuberculosis strain H83

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: B0W95_RS18955
• Protein ID: WP_003414166.1
• Coordinates: 3889305..3889661 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: B0W95_RS18960
• Protein ID: WP_003901465.1
• Coordinates: 3889645..3889875 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0W95_RS18935	3884757..3886445	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
B0W95_RS18940	3886449..3886775	-	327	WP_003414157.1	hypothetical protein	-
B0W95_RS18945	3886906..3887535	+	630	WP_003414158.1	DUF3558 family protein	-
B0W95_RS18950	3887554..3889203	+	1650	WP_003900576.1	CocE/NonD family hydrolase	-
B0W95_RS18955	3889305..3889661	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
B0W95_RS18960	3889645..3889875	-	231	WP_003901465.1	antitoxin MazE	Antitoxin
B0W95_RS18965	3889918..3890961	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
B0W95_RS18970	3891152..3891427	+	276	WP_003911993.1	DUF1778 domain-containing protein	-
B0W95_RS21590	3891603..3891857	-	255	WP_003917684.1	hypothetical protein	-
B0W95_RS18980	3892005..3892409	+	405	WP_009938577.1	hypothetical protein	-
B0W95_RS18985	3892406..3892597	+	192	WP_003414184.1	hypothetical protein	-
B0W95_RS18990	3892796..3893950	+	1155	WP_003899488.1	DDE-type integrase/transposase/recombinase	-
B0W95_RS18995	3894184..3894441	+	258	WP_003899489.1	hypothetical protein	-
B0W95_RS19000	3894546..3894857	+	312	WP_003414190.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3879340..3919910	40570

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T72930 WP_003414166.1 NZ_CP019611:c3889661-3889305 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T72930 NZ_CP019611:c3889661-3889305 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT72930 WP_003901465.1 NZ_CP019611:c3889875-3889645 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT72930 NZ_CP019611:c3889875-3889645 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• AlphaFold DB: A0A7U4FBW3