Detailed information of TA system TA072929

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3849721..3850408
• Replicon: chromosome
• Accession: NZ_CP019611
• Organism: Mycobacterium tuberculosis strain H83

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: B0W95_RS18740
• Protein ID: WP_003414064.1
• Coordinates: 3850013..3850408 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: B0W95_RS18735
• Protein ID: WP_003414061.1
• Coordinates: 3849721..3849987 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0W95_RS18710	3845360..3846262	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
B0W95_RS18715	3846331..3847083	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
B0W95_RS18720	3847327..3847602	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
B0W95_RS18725	3847599..3849221	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
B0W95_RS18730	3849308..3849724	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
B0W95_RS18735	3849721..3849987	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
B0W95_RS18740	3850013..3850408	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
B0W95_RS18745	3850405..3850674	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
B0W95_RS18750	3850684..3851778	-	1095	WP_003900565.1	restriction endonuclease subunit S	-
B0W95_RS18755	3851775..3852194	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
B0W95_RS18760	3852268..3852747	-	480	WP_003414073.1	dihydrofolate reductase	-
B0W95_RS18765	3852818..3853618	-	801	WP_003911953.1	thymidylate synthase	-
B0W95_RS18770	3853774..3854511	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T72929 WP_003414064.1 NZ_CP019611:c3850408-3850013 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T72929 NZ_CP019611:c3850408-3850013 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT72929 WP_003414061.1 NZ_CP019611:c3849987-3849721 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT72929 NZ_CP019611:c3849987-3849721 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64