Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3650016..3650653 Replicon chromosome
Accession NZ_CP019611
Organism Mycobacterium tuberculosis strain H83

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag B0W95_RS17600 Protein ID WP_003413180.1
Coordinates 3650016..3650411 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag B0W95_RS17605 Protein ID WP_003413183.1
Coordinates 3650408..3650653 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0W95_RS17555 3645419..3646630 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
B0W95_RS17565 3646757..3647416 + 660 WP_003899369.1 LppA family lipoprotein -
B0W95_RS17570 3647413..3648075 + 663 WP_003899370.1 hypothetical protein -
B0W95_RS17575 3648072..3648350 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
B0W95_RS17580 3648443..3648856 + 414 WP_003413164.1 PIN domain nuclease -
B0W95_RS17585 3648895..3649152 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
B0W95_RS17590 3649149..3649526 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
B0W95_RS17595 3649542..3649916 - 375 WP_003413177.1 hypothetical protein -
B0W95_RS17600 3650016..3650411 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
B0W95_RS17605 3650408..3650653 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
B0W95_RS17615 3651064..3651483 - 420 WP_003413190.1 A24 family peptidase -
B0W95_RS17620 3651495..3652304 - 810 WP_003413193.1 shikimate dehydrogenase -
B0W95_RS17625 3652301..3653554 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
B0W95_RS17630 3653547..3654059 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T72925 WP_003413180.1 NZ_CP019611:c3650411-3650016 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T72925 NZ_CP019611:c3650411-3650016 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT72925 WP_003413183.1 NZ_CP019611:c3650653-3650408 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT72925 NZ_CP019611:c3650653-3650408 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References