Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3045554..3046195 Replicon chromosome
Accession NZ_CP019611
Organism Mycobacterium tuberculosis strain H83

Toxin (Protein)


Gene name vapC Uniprot ID P64926
Locus tag B0W95_RS14645 Protein ID WP_003410075.1
Coordinates 3045797..3046195 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WLM7
Locus tag B0W95_RS14640 Protein ID WP_003899127.1
Coordinates 3045554..3045796 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0W95_RS14630 3043608..3043952 - 345 WP_003410065.1 ferredoxin family protein -
B0W95_RS14635 3044141..3045466 - 1326 WP_003410070.1 DUF4143 domain-containing protein -
B0W95_RS14640 3045554..3045796 + 243 WP_003899127.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
B0W95_RS14645 3045797..3046195 + 399 WP_003410075.1 PIN domain nuclease Toxin
B0W95_RS14650 3046378..3046809 - 432 WP_003410078.1 MarR family winged helix-turn-helix transcriptional regulator -
B0W95_RS14655 3046850..3047344 + 495 WP_003410080.1 DUF1398 domain-containing protein -
B0W95_RS14670 3047781..3047960 - 180 Protein_2779 hypothetical protein -
B0W95_RS14675 3048021..3048668 + 648 WP_071854215.1 transposase -
B0W95_RS14680 3048622..3049212 + 591 WP_003899131.1 IS110 family transposase -
B0W95_RS14685 3049340..3050596 - 1257 WP_003410095.1 HNH endonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-120)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(7-48)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14731.02 Da        Isoelectric Point: 6.7067

>T72916 WP_003410075.1 NZ_CP019611:3045797-3046195 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Download         Length: 399 bp

>T72916 NZ_CP019611:3045797-3046195 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8851.73 Da        Isoelectric Point: 4.1621

>AT72916 WP_003899127.1 NZ_CP019611:3045554-3045796 [Mycobacterium tuberculosis]
VYSGVVSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Download         Length: 243 bp

>AT72916 NZ_CP019611:3045554-3045796 [Mycobacterium tuberculosis]
GTGTATAGTGGTGTTGTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACT
CGATTCCAAGCGAAGTGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGC
TGCAGGGCAGCGGTTTCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCG
TAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4CHG

Antitoxin

Source ID Structure
PDB 4CHG
AlphaFold DB A0A045JPF9

References