Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3021829..3022415 Replicon chromosome
Accession NZ_CP019611
Organism Mycobacterium tuberculosis strain H83

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag B0W95_RS14545 Protein ID WP_003410010.1
Coordinates 3021829..3022173 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag B0W95_RS14550 Protein ID WP_003410014.1
Coordinates 3022167..3022415 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0W95_RS14505 3017535..3018134 + 600 WP_003409989.1 L-lysine exporter -
B0W95_RS14510 3018550..3018978 + 429 WP_003409992.1 cellulose-binding protein -
B0W95_RS14515 3019204..3019743 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
B0W95_RS14525 3020263..3020823 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
B0W95_RS14530 3020820..3021161 - 342 WP_003410003.1 DUF2384 domain-containing protein -
B0W95_RS14535 3021247..3021504 + 258 WP_003410006.1 hypothetical protein -
B0W95_RS14540 3021405..3021740 - 336 WP_003410009.1 dehydrogenase -
B0W95_RS14545 3021829..3022173 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
B0W95_RS14550 3022167..3022415 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
B0W95_RS14555 3022515..3024830 - 2316 WP_003899120.1 cation transporter ATPase CptG -
B0W95_RS14560 3024827..3025099 - 273 WP_003410017.1 DUF1490 family protein -
B0W95_RS14565 3025152..3025508 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
B0W95_RS14570 3025665..3026432 + 768 WP_003900447.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T72915 WP_003410010.1 NZ_CP019611:c3022173-3021829 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T72915 NZ_CP019611:c3022173-3021829 [Mycobacterium tuberculosis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT72915 WP_003410014.1 NZ_CP019611:c3022415-3022167 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT72915 NZ_CP019611:c3022415-3022167 [Mycobacterium tuberculosis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References