Detailed information of TA system TA072910

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2982167..2982870
• Replicon: chromosome
• Accession: NZ_CP019611
• Organism: Mycobacterium tuberculosis strain H83

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: B0W95_RS14255
• Protein ID: WP_003409778.1
• Coordinates: 2982167..2982496 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: B0W95_RS14260
• Protein ID: WP_003409780.1
• Coordinates: 2982493..2982870 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0W95_RS14235	2978550..2979620	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
B0W95_RS14240	2979617..2980132	+	516	WP_003409718.1	flavin reductase family protein	-
B0W95_RS14245	2980129..2981190	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
B0W95_RS14250	2981187..2981957	+	771	WP_003409775.1	SDR family oxidoreductase	-
B0W95_RS14255	2982167..2982496	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B0W95_RS14260	2982493..2982870	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
B0W95_RS14265	2982867..2983457	-	591	WP_003409784.1	SEC-C domain-containing protein	-
B0W95_RS14270	2983512..2984876	+	1365	WP_014585532.1	HNH endonuclease	-
B0W95_RS14275	2985031..2985483	-	453	WP_003899095.1	lipoprotein	-
B0W95_RS14280	2985547..2985948	+	402	WP_003409869.1	hypothetical protein	-
B0W95_RS14285	2985941..2986123	-	183	WP_003409870.1	hypothetical protein	-
B0W95_RS14290	2986237..2986587	-	351	WP_003899096.1	hypothetical protein	-
B0W95_RS14295	2986598..2987500	-	903	WP_003899097.1	hypothetical protein	-
B0W95_RS14300	2987521..2987712	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T72910 WP_003409778.1 NZ_CP019611:c2982496-2982167 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T72910 NZ_CP019611:c2982496-2982167 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT72910 WP_003409780.1 NZ_CP019611:c2982870-2982493 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT72910 NZ_CP019611:c2982870-2982493 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8