Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 2553267..2553895 Replicon chromosome
Accession NZ_CP019611
Organism Mycobacterium tuberculosis strain H83

Toxin (Protein)


Gene name vapC Uniprot ID P64880
Locus tag B0W95_RS12300 Protein ID WP_003407786.1
Coordinates 2553491..2553895 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WLU3
Locus tag B0W95_RS12295 Protein ID WP_003407785.1
Coordinates 2553267..2553485 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0W95_RS12265 2548687..2549067 + 381 WP_003407768.1 fumarate reductase subunit C -
B0W95_RS12270 2549064..2549441 + 378 WP_003407771.1 fumarate reductase subunit FrdD -
B0W95_RS12275 2549509..2550117 + 609 WP_003407773.1 TetR/AcrR family transcriptional regulator -
B0W95_RS12280 2550256..2551449 + 1194 WP_003901197.1 MMPL family transporter -
B0W95_RS12285 2551459..2551905 + 447 WP_003407780.1 F420H(2)-dependent quinone reductase -
B0W95_RS12290 2551940..2553229 + 1290 WP_003407781.1 threonine ammonia-lyase -
B0W95_RS12295 2553267..2553485 + 219 WP_003407785.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
B0W95_RS12300 2553491..2553895 + 405 WP_003407786.1 type II toxin-antitoxin system toxin ribonuclease VapC11 Toxin
B0W95_RS12305 2553912..2555654 - 1743 WP_003407788.1 malto-oligosyltrehalose trehalohydrolase -
B0W95_RS12310 2555647..2557944 - 2298 WP_003407790.1 malto-oligosyltrehalose synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(7-49)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14650.76 Da        Isoelectric Point: 5.6587

>T72907 WP_003407786.1 NZ_CP019611:2553491-2553895 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Download         Length: 405 bp

>T72907 NZ_CP019611:2553491-2553895 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8209.32 Da        Isoelectric Point: 4.5600

>AT72907 WP_003407785.1 NZ_CP019611:2553267-2553485 [Mycobacterium tuberculosis]
VYRWCMSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Download         Length: 219 bp

>AT72907 NZ_CP019611:2553267-2553485 [Mycobacterium tuberculosis]
GTGTATCGTTGGTGTATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCT
GACCACCAAGAGGGCGGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGC
TGGAAGGCGTCGGCTGGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6A7V

Antitoxin

Source ID Structure
PDB 6A7V
AlphaFold DB P9WLU3

References