Detailed information of TA system TA072902

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 2030643..2031211
• Replicon: chromosome
• Accession: NZ_CP019611
• Organism: Mycobacterium tuberculosis strain H83

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: B0W95_RS09840
• Protein ID: WP_003405865.1
• Coordinates: 2030837..2031211 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: B0W95_RS09835
• Protein ID: WP_003405863.1
• Coordinates: 2030643..2030840 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0W95_RS09815	2026684..2027322	-	639	WP_003898730.1	lipid droplet-associated protein	-
B0W95_RS09820	2027412..2028419	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
B0W95_RS09825	2028436..2029419	-	984	WP_003898731.1	hypothetical protein	-
B0W95_RS09830	2029482..2030555	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
B0W95_RS09835	2030643..2030840	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
B0W95_RS09840	2030837..2031211	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
B0W95_RS09845	2031414..2032112	+	699	WP_003898733.1	hypothetical protein	-
B0W95_RS09850	2032230..2032415	+	186	WP_003901093.1	hypothetical protein	-
B0W95_RS09855	2032342..2032617	-	276	WP_003405867.1	hypothetical protein	-
B0W95_RS09860	2032860..2033183	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
B0W95_RS09865	2033198..2034058	-	861	WP_003898734.1	hypothetical protein	-
B0W95_RS09870	2034091..2034861	-	771	Protein_1871	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T72902 WP_003405865.1 NZ_CP019611:2030837-2031211 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T72902 NZ_CP019611:2030837-2031211 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT72902 WP_003405863.1 NZ_CP019611:2030643-2030840 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT72902 NZ_CP019611:2030643-2030840 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07