Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 1485228..1485874 Replicon chromosome
Accession NZ_CP019611
Organism Mycobacterium tuberculosis strain H83

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag B0W95_RS06905 Protein ID WP_003403122.1
Coordinates 1485228..1485620 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag B0W95_RS06910 Protein ID WP_003403125.1
Coordinates 1485617..1485874 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0W95_RS06890 1480889..1482325 + 1437 WP_003900182.1 virulence factor Mce family protein -
B0W95_RS06895 1482413..1483621 + 1209 WP_003403114.1 virulence factor Mce family protein -
B0W95_RS06900 1483626..1485176 + 1551 WP_003403119.1 MCE family protein -
B0W95_RS06905 1485228..1485620 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
B0W95_RS06910 1485617..1485874 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
B0W95_RS06915 1486057..1487292 - 1236 WP_003403128.1 ATP-binding protein -
B0W95_RS06920 1487543..1487956 - 414 WP_003403137.1 type II toxin-antitoxin system VapC family toxin -
B0W95_RS06925 1487953..1488189 - 237 WP_003403139.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
B0W95_RS06930 1488293..1488799 - 507 WP_003900973.1 two component system sensor kinase HK1 -
B0W95_RS06935 1488913..1489383 - 471 WP_003403144.1 HAMP domain-containing protein -
B0W95_RS06940 1489427..1490188 - 762 WP_003403148.1 two-component system response regulator TcrA -
B0W95_RS06945 1490245..1490556 + 312 WP_003403164.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T72887 WP_003403122.1 NZ_CP019611:c1485620-1485228 [Mycobacterium tuberculosis]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T72887 NZ_CP019611:c1485620-1485228 [Mycobacterium tuberculosis]
GTGAACGTACGGCGCGCCCTCGCCGACACATCCGTTTTCATTGGCATCGAGGCGACACGATTCGACCCGGACCGGTTTGC
GGGATACGAATGGGGCGTCTCGGTAGTCACACTCGGCGAGCTGCGGCTCGGGGTGTTGCAAGCCTCCGGCCCCGAGGCCG
CCGCACGTCGGCTCTCCACCTACCAGCTTGCGCAACGGTTCGAACCACTAGGCATCGACGAGGCGGTCTCCGAAGCATGG
GCATTACTGGTGTCCAAGCTGCGCGCCGCCAAACTGCGCGTGCCGATCAACGACAGCTGGATTGCGGCCACAGCCGTGGC
GCACGGCATTGCGATCCTGACCCAAGACAACGACTACGCCGCCATGCCCGACGTCGAGGTCATAACGATCTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT72887 WP_003403125.1 NZ_CP019611:c1485874-1485617 [Mycobacterium tuberculosis]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT72887 NZ_CP019611:c1485874-1485617 [Mycobacterium tuberculosis]
ATGTCTGCTACGATACCCGCTCGCGATCTTCGCAACCACACCGCCGAGGTACTGCGGCGAGTTGCCGCCGGCGAGGAAAT
CGAGGTGCTCAAGGACAATCGCCCCGTAGCGCGCATCGTTCCGCTCAAGCGGCGCCGCCAATGGTTGCCAGCTGCCGAGG
TGATCGGCGAACTGGTGCGCTTGGGCCCCGATACCACCAATCTGGGCGAGGAGCTGCGAGAGACGCTGACGCAAACCACG
GACGATGTGCGGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References