Detailed information of TA system TA072873

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 4109657..4110297
• Replicon: chromosome
• Accession: NZ_CP019610
• Organism: Mycobacterium tuberculosis strain H54

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: B0W97_RS20090
• Protein ID: WP_003412970.1
• Coordinates: 4109878..4110297 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: B0W97_RS20085
• Protein ID: WP_003412975.1
• Coordinates: 4109657..4109881 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0W97_RS20065	4105274..4105837	+	564	WP_003412989.1	elongation factor P	-
B0W97_RS20070	4105840..4106310	+	471	WP_003899364.1	transcription antitermination factor NusB	-
B0W97_RS20075	4106310..4106711	+	402	WP_003412981.1	hypothetical protein	-
B0W97_RS20080	4106783..4109626	+	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
B0W97_RS20085	4109657..4109881	+	225	WP_003412975.1	antitoxin VapB39	Antitoxin
B0W97_RS20090	4109878..4110297	+	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
B0W97_RS20095	4110298..4111248	-	951	WP_003911916.1	Lsr2 family protein	-
B0W97_RS21690	4111307..4111552	+	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
B0W97_RS20100	4111893..4112813	+	921	WP_003412965.1	restriction endonuclease	-
B0W97_RS20105	4112848..4113249	-	402	WP_003412963.1	PIN domain-containing protein	-
B0W97_RS20110	4113246..4113468	-	223	Protein_3811	type II toxin-antitoxin system VapB family antitoxin	-
B0W97_RS20120	4113985..4114707	+	723	WP_003412957.1	DUF1906 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T72873 WP_003412970.1 NZ_CP019610:4109878-4110297 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T72873 NZ_CP019610:4109878-4110297 [Mycobacterium tuberculosis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT72873 WP_003412975.1 NZ_CP019610:4109657-4109881 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT72873 NZ_CP019610:4109657-4109881 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09