Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4095328..4095959 Replicon chromosome
Accession NZ_CP019610
Organism Mycobacterium tuberculosis strain H54

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag B0W97_RS19990 Protein ID WP_003413174.1
Coordinates 4095328..4095705 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag B0W97_RS19995 Protein ID WP_003413167.1
Coordinates 4095702..4095959 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0W97_RS19950 4090795..4091307 + 513 WP_003413197.1 Holliday junction resolvase RuvX -
B0W97_RS19955 4091300..4092553 + 1254 WP_003413196.1 endolytic transglycosylase MltG -
B0W97_RS19960 4092550..4093359 + 810 WP_003413193.1 shikimate dehydrogenase -
B0W97_RS19965 4093371..4093790 + 420 WP_003413190.1 A24 family peptidase -
B0W97_RS19975 4094201..4094446 + 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
B0W97_RS19980 4094443..4094838 + 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
B0W97_RS19985 4094938..4095312 + 375 WP_003413177.1 hypothetical protein -
B0W97_RS19990 4095328..4095705 - 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
B0W97_RS19995 4095702..4095959 - 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
B0W97_RS20000 4095998..4096411 - 414 WP_003413164.1 PIN domain nuclease -
B0W97_RS20005 4096504..4096781 - 278 Protein_3790 type II toxin-antitoxin system VapB family antitoxin -
B0W97_RS20010 4096778..4097440 - 663 WP_003905878.1 hypothetical protein -
B0W97_RS20015 4097437..4098096 - 660 WP_003902296.1 hypothetical protein -
B0W97_RS20025 4098223..4099434 - 1212 WP_003900845.1 alpha/beta hydrolase family protein -
B0W97_RS20030 4099730..4100137 - 408 WP_003413029.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T72871 WP_003413174.1 NZ_CP019610:c4095705-4095328 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T72871 NZ_CP019610:c4095705-4095328 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT72871 WP_003413167.1 NZ_CP019610:c4095959-4095702 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT72871 NZ_CP019610:c4095959-4095702 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References