Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 4094201..4094838 Replicon chromosome
Accession NZ_CP019610
Organism Mycobacterium tuberculosis strain H54

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag B0W97_RS19980 Protein ID WP_003413180.1
Coordinates 4094443..4094838 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag B0W97_RS19975 Protein ID WP_003413183.1
Coordinates 4094201..4094446 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0W97_RS19950 4090795..4091307 + 513 WP_003413197.1 Holliday junction resolvase RuvX -
B0W97_RS19955 4091300..4092553 + 1254 WP_003413196.1 endolytic transglycosylase MltG -
B0W97_RS19960 4092550..4093359 + 810 WP_003413193.1 shikimate dehydrogenase -
B0W97_RS19965 4093371..4093790 + 420 WP_003413190.1 A24 family peptidase -
B0W97_RS19975 4094201..4094446 + 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
B0W97_RS19980 4094443..4094838 + 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
B0W97_RS19985 4094938..4095312 + 375 WP_003413177.1 hypothetical protein -
B0W97_RS19990 4095328..4095705 - 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
B0W97_RS19995 4095702..4095959 - 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
B0W97_RS20000 4095998..4096411 - 414 WP_003413164.1 PIN domain nuclease -
B0W97_RS20005 4096504..4096781 - 278 Protein_3790 type II toxin-antitoxin system VapB family antitoxin -
B0W97_RS20010 4096778..4097440 - 663 WP_003905878.1 hypothetical protein -
B0W97_RS20015 4097437..4098096 - 660 WP_003902296.1 hypothetical protein -
B0W97_RS20025 4098223..4099434 - 1212 WP_003900845.1 alpha/beta hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T72870 WP_003413180.1 NZ_CP019610:4094443-4094838 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T72870 NZ_CP019610:4094443-4094838 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT72870 WP_003413183.1 NZ_CP019610:4094201-4094446 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT72870 NZ_CP019610:4094201-4094446 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References