Detailed information of TA system TA072867

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3894959..3895638
• Replicon: chromosome
• Accession: NZ_CP019610
• Organism: Mycobacterium tuberculosis strain H54

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: B0W97_RS18855
• Protein ID: WP_003414059.1
• Coordinates: 3895222..3895638 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: B0W97_RS18850
• Protein ID: WP_003414061.1
• Coordinates: 3894959..3895225 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0W97_RS18815	3890435..3891172	-	738	WP_003414079.1	dienelactone hydrolase family protein	-
B0W97_RS18820	3891328..3892128	+	801	WP_003911953.1	thymidylate synthase	-
B0W97_RS18825	3892199..3892678	+	480	WP_003414073.1	dihydrofolate reductase	-
B0W97_RS18830	3892752..3893171	+	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
B0W97_RS18835	3893168..3894262	+	1095	WP_003414068.1	restriction endonuclease subunit S	-
B0W97_RS18840	3894272..3894541	+	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
B0W97_RS18845	3894538..3894933	+	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
B0W97_RS18850	3894959..3895225	+	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
B0W97_RS18855	3895222..3895638	+	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
B0W97_RS18860	3895725..3897347	+	1623	WP_003414057.1	type I restriction-modification system subunit M	-
B0W97_RS18865	3897344..3897619	+	276	WP_003414055.1	type I restriction endonuclease subunit S	-
B0W97_RS18870	3897863..3898615	+	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
B0W97_RS18875	3898684..3899586	+	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T72867 WP_003414059.1 NZ_CP019610:3895222-3895638 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T72867 NZ_CP019610:3895222-3895638 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT72867 WP_003414061.1 NZ_CP019610:3894959-3895225 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT72867 NZ_CP019610:3894959-3895225 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64