Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3855071..3855641 Replicon chromosome
Accession NZ_CP019610
Organism Mycobacterium tuberculosis strain H54

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag B0W97_RS18630 Protein ID WP_003414166.1
Coordinates 3855285..3855641 (+) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag B0W97_RS18625 Protein ID WP_003901465.1
Coordinates 3855071..3855301 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0W97_RS18595 3851038..3852150 - 1113 WP_112668357.1 DDE-type integrase/transposase/recombinase -
B0W97_RS18600 3852349..3852540 - 192 WP_003414184.1 hypothetical protein -
B0W97_RS18605 3852537..3852941 - 405 WP_003414181.1 hypothetical protein -
B0W97_RS21685 3853089..3853343 + 255 WP_003917684.1 hypothetical protein -
B0W97_RS18615 3853519..3853794 - 276 WP_003911993.1 DUF1778 domain-containing protein -
B0W97_RS18620 3853985..3855028 + 1044 WP_003414172.1 DUF2293 domain-containing protein -
B0W97_RS18625 3855071..3855301 + 231 WP_003901465.1 antitoxin MazE Antitoxin
B0W97_RS18630 3855285..3855641 + 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
B0W97_RS18635 3855743..3857392 - 1650 WP_003899485.1 CocE/NonD family hydrolase -
B0W97_RS18640 3857411..3858040 - 630 WP_003414158.1 DUF3558 family protein -
B0W97_RS18645 3858171..3858497 + 327 WP_003414157.1 hypothetical protein -
B0W97_RS18650 3858501..3860189 + 1689 WP_003414155.1 alpha/beta hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T72865 WP_003414166.1 NZ_CP019610:3855285-3855641 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T72865 NZ_CP019610:3855285-3855641 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT72865 WP_003901465.1 NZ_CP019610:3855071-3855301 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT72865 NZ_CP019610:3855071-3855301 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References