Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3255927..3256601 Replicon chromosome
Accession NZ_CP019610
Organism Mycobacterium tuberculosis strain H54

Toxin (Protein)


Gene name vapC Uniprot ID P9WF52
Locus tag B0W97_RS15740 Protein ID WP_003417282.1
Coordinates 3256173..3256601 (+) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TI59
Locus tag B0W97_RS15735 Protein ID WP_003417286.1
Coordinates 3255927..3256169 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0W97_RS15710 3252463..3253527 + 1065 WP_003913037.1 GTP 3',8-cyclase MoaA -
B0W97_RS15715 3253624..3253998 + 375 WP_003417295.1 4a-hydroxytetrahydrobiopterin dehydratase -
B0W97_RS15720 3253995..3254528 + 534 WP_003417293.1 cyclic pyranopterin monophosphate synthase MoaC -
B0W97_RS15725 3254529..3255194 + 666 WP_003417290.1 molybdopterin converting factor subunit 1 -
B0W97_RS15730 3255191..3255805 + 615 WP_003417288.1 class I SAM-dependent methyltransferase -
B0W97_RS15735 3255927..3256169 + 243 WP_003417286.1 ribbon-helix-helix domain-containing protein Antitoxin
B0W97_RS15740 3256173..3256601 + 429 WP_003417282.1 type II toxin-antitoxin system VapC family toxin Toxin
B0W97_RS15745 3256680..3257471 - 792 WP_003417279.1 succinate dehydrogenase iron-sulfur subunit -
B0W97_RS15750 3257471..3259243 - 1773 WP_003417273.1 succinate dehydrogenase flavoprotein subunit -
B0W97_RS15755 3259372..3259806 - 435 WP_003902441.1 succinate dehydrogenase hydrophobic membrane anchor subunit -
B0W97_RS15760 3259803..3260237 - 435 WP_003900017.1 succinate dehydrogenase, cytochrome b556 subunit -
B0W97_RS15765 3260378..3260779 + 402 WP_003417264.1 cytidine deaminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-141)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15834.16 Da        Isoelectric Point: 8.5471

>T72859 WP_003417282.1 NZ_CP019610:3256173-3256601 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Download         Length: 429 bp

>T72859 NZ_CP019610:3256173-3256601 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8849.86 Da        Isoelectric Point: 5.1007

>AT72859 WP_003417286.1 NZ_CP019610:3255927-3256169 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Download         Length: 243 bp

>AT72859 NZ_CP019610:3255927-3256169 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBL0

Antitoxin

Source ID Structure
AlphaFold DB G0TI59

References