Detailed information of TA system TA072858

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/Txe-YefM
• Location: 3185583..3186112
• Replicon: chromosome
• Accession: NZ_CP019610
• Organism: Mycobacterium tuberculosis strain H54

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TI95
• Locus tag: B0W97_RS15480
• Protein ID: WP_003417760.1
• Coordinates: 3185583..3185840 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G0TI94
• Locus tag: B0W97_RS15485
• Protein ID: WP_003417757.1
• Coordinates: 3185837..3186112 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0W97_RS15450	3182011..3182403	+	393	WP_003417776.1	roadblock/LC7 domain-containing protein	-
B0W97_RS15455	3182381..3182749	+	369	WP_003417772.1	DUF742 domain-containing protein	-
B0W97_RS15460	3182730..3183311	+	582	WP_003417769.1	ATP/GTP-binding protein	-
B0W97_RS15465	3183318..3183869	+	552	WP_003417767.1	pentapeptide repeat protein MfpA	-
B0W97_RS15470	3183866..3184234	-	369	WP_003417765.1	FHA domain-containing protein	-
B0W97_RS15475	3184351..3185541	-	1191	WP_003900033.1	NADH:flavin oxidoreductase	-
B0W97_RS15480	3185583..3185840	-	258	WP_003417760.1	Txe/YoeB family addiction module toxin	Toxin
B0W97_RS15485	3185837..3186112	-	276	WP_003417757.1	type II toxin-antitoxin system antitoxin RelJ	Antitoxin
B0W97_RS15490	3186236..3187081	+	846	WP_003417751.1	bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase	-
B0W97_RS15495	3187078..3187371	+	294	WP_003417749.1	DUF3017 domain-containing protein	-
B0W97_RS15500	3187385..3187774	-	390	WP_003417745.1	DUF732 domain-containing protein	-
B0W97_RS15505	3187889..3188149	+	261	WP_003417741.1	hypothetical protein	-
B0W97_RS15510	3188176..3188394	+	219	WP_157132559.1	hypothetical protein	-
B0W97_RS15515	3188292..3188663	+	372	WP_003417739.1	FAD-binding oxidoreductase	-
B0W97_RS15520	3188745..3189539	+	795	WP_003417738.1	BBE domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-72)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10070.30 Da
• Isoelectric Point: 6.4693

>T72858 WP_003417760.1 NZ_CP019610:c3185840-3185583 [Mycobacterium tuberculosis]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Nucleotide

• Length: 258 bp

>T72858 NZ_CP019610:c3185840-3185583 [Mycobacterium tuberculosis]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTGGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.35 Da
• Isoelectric Point: 4.6652

>AT72858 WP_003417757.1 NZ_CP019610:c3186112-3185837 [Mycobacterium tuberculosis]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Nucleotide

• Length: 276 bp

>AT72858 NZ_CP019610:c3186112-3185837 [Mycobacterium tuberculosis]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Structures

Toxin

• PDB: 3OEI

Antitoxin

• PDB: 3CTO
• PDB: 3OEI
• PDB: 3D55
• AlphaFold DB: G0TI94