Detailed information of TA system TA072854

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2211362..2211996
• Replicon: chromosome
• Accession: NZ_CP019610
• Organism: Mycobacterium tuberculosis strain H54

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF84
• Locus tag: B0W97_RS10870
• Protein ID: WP_003401406.1
• Coordinates: 2211568..2211996 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: B0W97_RS10865
• Protein ID: WP_011961607.1
• Coordinates: 2211362..2211544 (+)
• Length: 61 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0W97_RS10860	2208460..2211267	+	2808	WP_025202582.1	PE family protein	-
B0W97_RS10865	2211362..2211544	+	183	WP_011961607.1	hypothetical protein	Antitoxin
B0W97_RS10870	2211568..2211996	+	429	WP_003401406.1	type II toxin-antitoxin system VapC family toxin	Toxin
B0W97_RS10875	2212036..2212956	-	921	WP_003401404.1	DUF2236 domain-containing protein	-
B0W97_RS10880	2213046..2213771	+	726	WP_003899886.1	TetR/AcrR family transcriptional regulator	-
B0W97_RS10885	2213701..2214282	-	582	WP_003401399.1	VOC family protein	-
B0W97_RS10890	2214379..2214999	+	621	WP_003401397.1	TetR/AcrR family transcriptional regulator	-
B0W97_RS10895	2214996..2216129	+	1134	WP_003401394.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-133)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15887.27 Da
• Isoelectric Point: 7.2214

>T72854 WP_003401406.1 NZ_CP019610:2211568-2211996 [Mycobacterium tuberculosis]
MFLIDVNVLLAAHRGDHPNHRTVRPWFDRLLAADDPFTVPNLVWASFLRLTTNRRIFEIPSPRADAFAFVEAVNAQPHHL
PTSPGPRHLVLLRKLCDEADASGDLIPDAVLGAIAVEHHCAVVSLDRDFARFASVRHIRPPI

Nucleotide

• Length: 429 bp

>T72854 NZ_CP019610:2211568-2211996 [Mycobacterium tuberculosis]
ATGTTCCTCATCGACGTCAACGTGCTGCTGGCTGCACACCGCGGTGACCATCCGAATCACCGAACCGTCCGCCCCTGGTT
CGATCGACTGCTTGCGGCCGACGACCCCTTCACAGTGCCGAACCTAGTGTGGGCGTCGTTCCTCCGGCTAACAACGAATC
GACGCATCTTCGAGATTCCGTCACCACGAGCGGATGCATTCGCATTCGTCGAAGCCGTCAACGCCCAGCCCCATCACCTT
CCGACAAGCCCCGGCCCCAGACACCTGGTGCTGCTGCGAAAACTCTGCGACGAGGCCGACGCATCGGGCGACTTGATACC
TGACGCGGTACTCGGGGCCATCGCCGTGGAGCATCACTGCGCCGTGGTGAGCCTGGATAGGGATTTCGCCCGGTTTGCCT
CTGTGCGCCATATTCGCCCGCCAATCTAG

Antitoxin

Protein

• Length: 61 a.a.
• Molecular weight: 6262.06 Da
• Isoelectric Point: 4.5878

>AT72854 WP_011961607.1 NZ_CP019610:2211362-2211544 [Mycobacterium tuberculosis]
MGAVIEDALRRELAAARTGGARPTVPVFDAGTGPRPGIDLTSNTVLSEVLDEGLELNSRK

Nucleotide

• Length: 183 bp

>AT72854 NZ_CP019610:2211362-2211544 [Mycobacterium tuberculosis]
CTGGGCGCTGTGATCGAAGACGCCCTTCGGCGGGAGCTCGCCGCCGCCCGTACCGGCGGCGCCCGGCCAACCGTCCCCGT
TTTTGACGCCGGCACCGGTCCGCGGCCAGGTATCGACCTAACCTCGAATACGGTGTTGTCCGAAGTGCTCGACGAGGGTC
TGGAACTGAACTCGCGGAAGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BRM6