Detailed information of TA system TA072852

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 2180565..2181136
• Replicon: chromosome
• Accession: NZ_CP019610
• Organism: Mycobacterium tuberculosis strain H54

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: B0W97_RS10750
• Protein ID: WP_003401560.1
• Coordinates: 2180834..2181136 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: B0W97_RS10745
• Protein ID: WP_003401563.1
• Coordinates: 2180565..2180786 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0W97_RS10730	2178470..2179378	-	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-
B0W97_RS10735	2179375..2180007	-	633	WP_003905275.1	TetR/AcrR family transcriptional regulator	-
B0W97_RS10740	2180143..2180568	-	426	WP_003401566.1	PIN domain nuclease	-
B0W97_RS10745	2180565..2180786	-	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
B0W97_RS10750	2180834..2181136	-	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
B0W97_RS10755	2181133..2181360	-	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
B0W97_RS10760	2181503..2183278	-	1776	WP_010886074.1	PE family protein	-
B0W97_RS10765	2183457..2184854	+	1398	WP_003401544.1	sulfatase	-
B0W97_RS10770	2184864..2185667	+	804	WP_003401540.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T72852 WP_003401560.1 NZ_CP019610:c2181136-2180834 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T72852 NZ_CP019610:c2181136-2180834 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT72852 WP_003401563.1 NZ_CP019610:c2180786-2180565 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT72852 NZ_CP019610:c2180786-2180565 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• PDB: 3H87
• AlphaFold DB: A0A829CG48