Detailed information of TA system TA072849

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 1903745..1904421
• Replicon: chromosome
• Accession: NZ_CP019610
• Organism: Mycobacterium tuberculosis strain H54

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: B0W97_RS09440
• Protein ID: WP_003898500.1
• Coordinates: 1904008..1904421 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: B0W97_RS09435
• Protein ID: WP_003402915.1
• Coordinates: 1903745..1904011 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0W97_RS09420	1899179..1900159	-	981	WP_003402923.1	o-succinylbenzoate synthase	-
B0W97_RS09425	1900156..1901760	-	1605	WP_003402920.1	amidohydrolase	-
B0W97_RS09430	1901838..1903553	+	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
B0W97_RS09435	1903745..1904011	+	267	WP_003402915.1	antitoxin VapB3	Antitoxin
B0W97_RS09440	1904008..1904421	+	414	WP_003898500.1	PIN domain-containing protein	Toxin
B0W97_RS09445	1904735..1905637	+	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
B0W97_RS09450	1905733..1906617	+	885	WP_003402909.1	SDR family oxidoreductase	-
B0W97_RS09455	1906680..1907066	+	387	WP_003402907.1	VOC family protein	-
B0W97_RS09460	1907185..1908438	+	1254	WP_003402904.1	inorganic phosphate transporter family protein	-
B0W97_RS09465	1908435..1908713	+	279	WP_003402901.1	hypothetical protein	-
B0W97_RS09470	1908773..1909075	+	303	WP_003402896.1	DUF3349 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T72849 WP_003898500.1 NZ_CP019610:1904008-1904421 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T72849 NZ_CP019610:1904008-1904421 [Mycobacterium tuberculosis]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT72849 WP_003402915.1 NZ_CP019610:1903745-1904011 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT72849 NZ_CP019610:1903745-1904011 [Mycobacterium tuberculosis]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0