Detailed information of TA system TA072846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 1846855..1847501
• Replicon: chromosome
• Accession: NZ_CP019610
• Organism: Mycobacterium tuberculosis strain H54

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: B0W97_RS09175
• Protein ID: WP_003403137.1
• Coordinates: 1847088..1847501 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: B0W97_RS09170
• Protein ID: WP_003403139.1
• Coordinates: 1846855..1847091 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0W97_RS09135	1841896..1842639	-	744	WP_003403177.1	IS607 family element transposase accessory protein TnpB	-
B0W97_RS09140	1842641..1843225	-	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
B0W97_RS09145	1843466..1844416	-	951	WP_003900184.1	DUF1259 domain-containing protein	-
B0W97_RS09150	1844488..1844799	-	312	WP_003403164.1	hypothetical protein	-
B0W97_RS09155	1844856..1845617	+	762	WP_003403148.1	two-component system response regulator TcrA	-
B0W97_RS09160	1845661..1846131	+	471	WP_003403144.1	HAMP domain-containing protein	-
B0W97_RS09165	1846245..1846751	+	507	WP_003900973.1	two component system sensor kinase HK1	-
B0W97_RS09170	1846855..1847091	+	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
B0W97_RS09175	1847088..1847501	+	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
B0W97_RS09180	1847752..1848987	+	1236	WP_003403128.1	ATP-binding protein	-
B0W97_RS09185	1849170..1849427	+	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
B0W97_RS09190	1849424..1849816	+	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
B0W97_RS09195	1849868..1851418	-	1551	WP_003905349.1	MCE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T72846 WP_003403137.1 NZ_CP019610:1847088-1847501 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T72846 NZ_CP019610:1847088-1847501 [Mycobacterium tuberculosis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT72846 WP_003403139.1 NZ_CP019610:1846855-1847091 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT72846 NZ_CP019610:1846855-1847091 [Mycobacterium tuberculosis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89