Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1313076..1313707 Replicon chromosome
Accession NZ_CP019610
Organism Mycobacterium tuberculosis strain H54

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag B0W97_RS06305 Protein ID WP_003405820.1
Coordinates 1313396..1313707 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag B0W97_RS06300 Protein ID WP_003405836.1
Coordinates 1313076..1313396 (+) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B0W97_RS06275 1308277..1308915 + 639 WP_003898730.1 lipid droplet-associated protein -
B0W97_RS06280 1308912..1310159 + 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
B0W97_RS06285 1310149..1310406 + 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
B0W97_RS06290 1310416..1311528 + 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
B0W97_RS06295 1311535..1313066 - 1532 Protein_1204 carboxylesterase/lipase family protein -
B0W97_RS06300 1313076..1313396 + 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
B0W97_RS06305 1313396..1313707 + 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
B0W97_RS06310 1313819..1314976 + 1158 WP_003898727.1 AI-2E family transporter -
B0W97_RS06315 1314983..1315684 - 702 WP_003405815.1 DUF4245 domain-containing protein -
B0W97_RS06320 1315683..1316771 + 1089 WP_003898726.1 class II fructose-bisphosphatase -
B0W97_RS06325 1316802..1318226 + 1425 WP_003405805.1 class II fumarate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T72834 WP_003405820.1 NZ_CP019610:1313396-1313707 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T72834 NZ_CP019610:1313396-1313707 [Mycobacterium tuberculosis]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT72834 WP_003405836.1 NZ_CP019610:1313076-1313396 [Mycobacterium tuberculosis]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT72834 NZ_CP019610:1313076-1313396 [Mycobacterium tuberculosis]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References