Detailed information of TA system TA072833

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1304388..1304956
• Replicon: chromosome
• Accession: NZ_CP019610
• Organism: Mycobacterium tuberculosis strain H54

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: B0W97_RS06250
• Protein ID: WP_003405865.1
• Coordinates: 1304388..1304762 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: B0W97_RS06255
• Protein ID: WP_003405863.1
• Coordinates: 1304759..1304956 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0W97_RS06220	1300738..1301508	+	771	Protein_1189	adenylate/guanylate cyclase domain-containing protein	-
B0W97_RS06225	1301541..1302401	+	861	WP_003898734.1	hypothetical protein	-
B0W97_RS06230	1302416..1302739	-	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
B0W97_RS06235	1302982..1303257	+	276	WP_031651777.1	hypothetical protein	-
B0W97_RS06240	1303184..1303369	-	186	WP_003901093.1	hypothetical protein	-
B0W97_RS06245	1303487..1304185	-	699	WP_003898733.1	hypothetical protein	-
B0W97_RS06250	1304388..1304762	-	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
B0W97_RS06255	1304759..1304956	-	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
B0W97_RS06260	1305044..1306117	-	1074	WP_003898732.1	redox-regulated ATPase YchF	-
B0W97_RS06265	1306180..1307163	+	984	WP_003898731.1	hypothetical protein	-
B0W97_RS06270	1307180..1308187	-	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
B0W97_RS06275	1308277..1308915	+	639	WP_003898730.1	lipid droplet-associated protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T72833 WP_003405865.1 NZ_CP019610:c1304762-1304388 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T72833 NZ_CP019610:c1304762-1304388 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT72833 WP_003405863.1 NZ_CP019610:c1304956-1304759 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT72833 NZ_CP019610:c1304956-1304759 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07