Detailed information of TA system TA072825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 361377..362080
• Replicon: chromosome
• Accession: NZ_CP019610
• Organism: Mycobacterium tuberculosis strain H54

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: B0W97_RS01870
• Protein ID: WP_003409778.1
• Coordinates: 361751..362080 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: B0W97_RS01865
• Protein ID: WP_003409780.1
• Coordinates: 361377..361754 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0W97_RS01825	356535..356726	+	192	WP_003409876.1	hypothetical protein	-
B0W97_RS01830	356747..357649	+	903	WP_003899097.1	hypothetical protein	-
B0W97_RS01835	357660..358010	+	351	WP_003409871.1	hypothetical protein	-
B0W97_RS01840	358124..358306	+	183	WP_003409870.1	hypothetical protein	-
B0W97_RS01845	358299..358700	-	402	WP_003409869.1	hypothetical protein	-
B0W97_RS01850	358764..359216	+	453	WP_003899095.1	lipoprotein	-
B0W97_RS01855	359371..360735	-	1365	WP_003903691.1	HNH endonuclease	-
B0W97_RS01860	360790..361380	+	591	WP_003409784.1	SEC-C domain-containing protein	-
B0W97_RS01865	361377..361754	+	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
B0W97_RS01870	361751..362080	+	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B0W97_RS01875	362290..363060	-	771	WP_003409775.1	SDR family oxidoreductase	-
B0W97_RS01880	363057..364118	-	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
B0W97_RS01885	364115..364630	-	516	WP_003409718.1	flavin reductase family protein	-
B0W97_RS01890	364627..365697	-	1071	WP_003902252.1	epoxide hydrolase EphB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T72825 WP_003409778.1 NZ_CP019610:361751-362080 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T72825 NZ_CP019610:361751-362080 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT72825 WP_003409780.1 NZ_CP019610:361377-361754 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT72825 NZ_CP019610:361377-361754 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8