Detailed information of TA system TA072816

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 181013..181708
• Replicon: chromosome
• Accession: NZ_CP019610
• Organism: Mycobacterium tuberculosis strain H54

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: B0W97_RS00925
• Protein ID: WP_003905754.1
• Coordinates: 181274..181708 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TMN0
• Locus tag: B0W97_RS00920
• Protein ID: WP_003410814.1
• Coordinates: 181013..181267 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B0W97_RS00890	176066..176812	+	747	WP_003901330.1	proteasome subunit alpha	-
B0W97_RS00895	177352..178083	-	732	WP_003900467.1	PPE family protein	-
B0W97_RS00900	178139..178435	-	297	WP_003410820.1	PE family protein	-
B0W97_RS00915	180740..180997	+	258	WP_003410816.1	hypothetical protein	-
B0W97_RS00920	181013..181267	+	255	WP_003410814.1	antitoxin	Antitoxin
B0W97_RS00925	181274..181708	+	435	WP_003905754.1	type II toxin-antitoxin system VapC family toxin	Toxin
B0W97_RS00930	181687..182403	-	717	WP_003410793.1	SWIM zinc finger family protein	-
B0W97_RS00935	182513..185554	-	3042	WP_003899171.1	DEAD/DEAH box helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15632.05 Da
• Isoelectric Point: 7.4681

>T72816 WP_003905754.1 NZ_CP019610:181274-181708 [Mycobacterium tuberculosis]
MKIVDANVLLYAVNTASEHHKPSLRWLDGALSGADRVGFAWVPLLAFVRLATKVGLFPRPLPREAAITQVADWLAAPSAV
LVNPTVRHADILARMLTYVGTGANLVNDAHLAALAVEHRASIVSYDSDFGRFEGVRWDQPPALL

Nucleotide

• Length: 435 bp

>T72816 NZ_CP019610:181274-181708 [Mycobacterium tuberculosis]
ATGAAGATCGTCGACGCGAACGTCTTGCTCTACGCCGTGAACACCGCAAGTGAGCACCACAAGCCGTCGCTGCGCTGGCT
TGACGGTGCGCTGTCGGGCGCCGACCGCGTCGGGTTCGCCTGGGTGCCGTTGTTGGCGTTCGTGCGATTGGCGACCAAGG
TGGGGTTGTTCCCCCGTCCGCTTCCGCGGGAGGCGGCCATCACCCAGGTCGCGGATTGGCTAGCCGCACCCAGCGCCGTC
TTGGTGAATCCGACCGTCCGGCACGCCGATATCCTGGCGAGAATGCTGACGTACGTGGGAACCGGTGCCAACCTGGTCAA
CGACGCGCATCTGGCCGCGCTTGCCGTCGAGCATCGCGCCAGCATCGTGTCCTACGACAGTGACTTCGGCCGATTCGAAG
GGGTGCGCTGGGATCAGCCGCCCGCGCTGTTGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9417.57 Da
• Isoelectric Point: 8.9912

>AT72816 WP_003410814.1 NZ_CP019610:181013-181267 [Mycobacterium tuberculosis]
MRTTVTLDDDVEQLVRRRMAERQVSFKKALNDAIRDGASGRPAPSHFSTRTADLGVPAVNLDRALQLAADLEDEELVRRQ
RRGS

Nucleotide

• Length: 255 bp

>AT72816 NZ_CP019610:181013-181267 [Mycobacterium tuberculosis]
ATGCGGACGACTGTCACATTGGATGACGACGTCGAGCAGTTGGTAAGGCGCCGGATGGCCGAGCGGCAAGTGTCCTTCAA
GAAGGCCCTCAACGACGCGATTCGCGACGGCGCCTCCGGGCGACCTGCGCCGAGTCACTTCAGCACCCGCACGGCAGACT
TGGGCGTCCCTGCGGTCAACCTCGACCGGGCCTTGCAGCTTGCGGCCGACCTCGAGGACGAAGAGCTTGTGCGCCGTCAA
CGCCGCGGGTCATAG

Structures

Antitoxin

• AlphaFold DB: G0TMN0