Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/YafQ-RelB
Location 1297004..1297570 Replicon chromosome
Accession NZ_CP019596
Organism Bifidobacterium breve strain LMC520

Toxin (Protein)


Gene name relE Uniprot ID A0A0L7CVW6
Locus tag BBL520_RS05905 Protein ID WP_052790763.1
Coordinates 1297277..1297570 (+) Length 98 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0L7CVI8
Locus tag BBL520_RS05900 Protein ID WP_052790764.1
Coordinates 1297004..1297273 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBL520_RS05875 1292756..1293451 - 696 WP_003829410.1 response regulator transcription factor -
BBL520_RS05880 1293577..1294092 + 516 WP_013140733.1 hypothetical protein -
BBL520_RS05885 1294089..1294979 + 891 WP_007056280.1 hypothetical protein -
BBL520_RS05890 1294976..1295656 + 681 WP_003829413.1 ABC transporter ATP-binding protein -
BBL520_RS05895 1295653..1296819 + 1167 WP_052790765.1 ABC transporter permease -
BBL520_RS05900 1297004..1297273 + 270 WP_052790764.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BBL520_RS05905 1297277..1297570 + 294 WP_052790763.1 type II toxin-antitoxin system YafQ family toxin Toxin
BBL520_RS10985 1297576..1297875 - 300 WP_052790762.1 hypothetical protein -
BBL520_RS05915 1298238..1298789 + 552 WP_052789591.1 RNA polymerase sigma factor -
BBL520_RS05920 1298823..1299650 + 828 WP_077149499.1 hypothetical protein -
BBL520_RS05925 1299781..1300407 + 627 WP_077149500.1 hypothetical protein -
BBL520_RS05930 1300404..1301186 + 783 WP_077149501.1 murein hydrolase transporter LrgB -
BBL520_RS05935 1301238..1302113 + 876 WP_077149502.1 ABC transporter ATP-binding protein -
BBL520_RS05940 1302168..1302566 + 399 WP_077149503.1 superoxide dismutase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1288885..1316435 27550


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-94)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(3-87)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 11218.94 Da        Isoelectric Point: 9.8306

>T72808 WP_052790763.1 NZ_CP019596:1297277-1297570 [Bifidobacterium breve]
MLKPDYTAAFQRDIKKLKRKHADLRPLKEVIRLVLEDTADSKEALRRRHRTHTLTGDLNGVLECHIGNAGDWLLLWIRDD
GTAMFMRTGSHDELLGR

Download         Length: 294 bp

>T72808 NZ_CP019596:1297277-1297570 [Bifidobacterium breve]
ATGCTCAAACCGGATTACACGGCGGCATTCCAGCGAGATATCAAGAAGCTCAAACGCAAGCACGCTGATTTACGCCCGCT
GAAAGAAGTCATTCGTCTGGTGCTTGAGGACACGGCGGACTCCAAGGAAGCATTGAGGCGTAGGCATCGCACCCACACAC
TTACCGGTGATCTAAACGGCGTACTGGAATGCCACATCGGCAATGCCGGCGACTGGCTGCTTCTATGGATTCGCGATGAT
GGTACCGCCATGTTCATGCGAACCGGCAGCCATGACGAACTGTTGGGCAGATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9832.11 Da        Isoelectric Point: 4.5742

>AT72808 WP_052790764.1 NZ_CP019596:1297004-1297273 [Bifidobacterium breve]
VSMASVTVRVDADTKQKAANIAEDLGLDLSSVTRAFYKQIVREKRIPLNLSYTTVPPETMEALDEAKNRLADGQPRFNSA
DEMFDSLGI

Download         Length: 270 bp

>AT72808 NZ_CP019596:1297004-1297273 [Bifidobacterium breve]
GTGAGCATGGCATCCGTCACAGTCCGCGTGGATGCGGACACCAAGCAGAAAGCCGCAAACATAGCCGAGGATCTCGGTCT
GGACTTGTCTTCGGTCACCCGCGCCTTCTACAAGCAGATTGTCCGGGAGAAGCGCATTCCCCTAAACCTTTCCTATACGA
CCGTGCCGCCTGAAACCATGGAGGCCCTTGACGAGGCGAAGAACAGGCTCGCCGACGGCCAACCTCGCTTCAACAGTGCC
GATGAGATGTTCGATTCGTTGGGAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7CVW6

Antitoxin

Source ID Structure
AlphaFold DB A0A0L7CVI8

References