Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/RelB(antitoxin)
Location 1066348..1066984 Replicon chromosome
Accession NZ_CP019596
Organism Bifidobacterium breve strain LMC520

Toxin (Protein)


Gene name mazF Uniprot ID D4BP71
Locus tag BBL520_RS04675 Protein ID WP_003829234.1
Coordinates 1066348..1066704 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID D6PAX1
Locus tag BBL520_RS04680 Protein ID WP_012577921.1
Coordinates 1066691..1066984 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BBL520_RS04645 1061540..1062448 + 909 WP_077149476.1 ABC transporter ATP-binding protein -
BBL520_RS04650 1062645..1063271 + 627 WP_003829226.1 30S ribosomal protein S4 -
BBL520_RS04655 1063608..1064390 - 783 WP_015438747.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
BBL520_RS04660 1064532..1065017 - 486 WP_003829229.1 cytidine deaminase -
BBL520_RS04665 1065108..1065464 + 357 WP_003833231.1 SdpI family protein -
BBL520_RS04670 1065569..1066171 + 603 WP_003829233.1 transglutaminase family protein -
BBL520_RS04675 1066348..1066704 - 357 WP_003829234.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BBL520_RS04680 1066691..1066984 - 294 WP_012577921.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BBL520_RS04685 1067135..1067299 - 165 Protein_890 IS30 family transposase -
BBL520_RS04690 1067399..1068073 + 675 WP_015438748.1 histidine phosphatase family protein -
BBL520_RS04695 1068156..1068539 + 384 WP_016463075.1 DUF948 domain-containing protein -
BBL520_RS04700 1068539..1068775 + 237 WP_003829239.1 hypothetical protein -
BBL520_RS04705 1068871..1071549 + 2679 WP_003829240.1 alanine--tRNA ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-110)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(11-53)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13316.23 Da        Isoelectric Point: 7.9984

>T72807 WP_003829234.1 NZ_CP019596:c1066704-1066348 [Bifidobacterium breve]
MMKTDPHQFEIWWVPFTFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSSLFLDDGPTGRLSAYDAGRVTWAINELYPGLLA

Download         Length: 357 bp

>T72807 NZ_CP019596:c1066704-1066348 [Bifidobacterium breve]
ATGATGAAGACTGATCCGCACCAGTTCGAGATATGGTGGGTGCCGTTCACTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAGTCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCACCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10866.31 Da        Isoelectric Point: 4.2896

>AT72807 WP_012577921.1 NZ_CP019596:c1066984-1066691 [Bifidobacterium breve]
MAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Download         Length: 294 bp

>AT72807 NZ_CP019596:c1066984-1066691 [Bifidobacterium breve]
ATGGCTATCAAGGAGAAGACCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7B6G7

Antitoxin

Source ID Structure
AlphaFold DB A0A1S2VTZ6

References